Anti-ALKBH8 antibody (ab113512)
Key features and details
- Rabbit polyclonal to ALKBH8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ALKBH8 antibody -
Description
Rabbit polyclonal to ALKBH8 -
Host species
Rabbit -
Specificity
ab113512 is also predicted to react with isoform 4 of Human ALKBH8 (76kDa). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to N terminal amino acids 2-238 of Human ALKBH8 (BC015183).
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Positive control
- Human fetal spleen and A549 cell lysates; Human fetal spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab113512 is purified by a protein affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. -
Tissue specificity
Widely expressed, with highest expression in spleen, followed by pancreas and lung. -
Sequence similarities
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 91801 Human
- Entrez Gene: 67667 Mouse
- Entrez Gene: 366783 Rat
- Omim: 613306 Human
- SwissProt: Q96BT7 Human
- SwissProt: Q80Y20 Mouse
- Unigene: 503763 Human
- Unigene: 116968 Mouse
see all -
Alternative names
- 4930562C03Rik antibody
- 8030431D03Rik antibody
- 9430088N01Rik antibody
see all
Images
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All lanes : Anti-ALKBH8 antibody (ab113512) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : A549 cell lysate
Predicted band size: 75 kDa -
ab113512 at 1/100 dilution staining ALKBH8 in Formalin-fixed, Paraffin-embedded Human fetal spleen tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab113512 has been referenced in 1 publication.
- Delaunay S et al. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. J Exp Med 213:2503-2523 (2016). PubMed: 27811057