Overview

  • Product name
  • Description
    Rabbit polyclonal to ALKBH8
  • Specificity
    ab113512 is also predicted to react with isoform 4 of Human ALKBH8 (76kDa).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to N terminal amino acids 2-238 of Human ALKBH8 (BC015183).

  • Positive control
    • Human fetal spleen and A549 cell lysates; Human fetal spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab113512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 75 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
  • Tissue specificity
    Widely expressed, with highest expression in spleen, followed by pancreas and lung.
  • Sequence similarities
    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930562C03Rik antibody
    • 8030431D03Rik antibody
    • 9430088N01Rik antibody
    • ABH8 antibody
    • alkB alkylation repair homolog 8 (E. coli) antibody
    • alkB alkylation repair homolog 8 antibody
    • AlkB homologue 8 antibody
    • ALKB8_HUMAN antibody
    • ALKBH8 antibody
    • Alkylated DNA repair protein alkB homolog 8 antibody
    • FLJ38204 antibody
    • MGC10235 antibody
    • OTTHUMP00000211286 antibody
    • OTTHUMP00000211287 antibody
    • Probable alpha-ketoglutarate-dependent dioxygenase ABH8 antibody
    • RGD1304687 antibody
    • S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8 antibody
    • tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 antibody
    see all

Images

  • All lanes : Anti-ALKBH8 antibody (ab113512) at 1/500 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : A549 cell lysate


    Predicted band size : 75 kDa
  • ab113512 at 1/100 dilution staining ALKBH8 in Formalin-fixed, Paraffin-embedded Human fetal spleen tissue by Immunohistochemistry.
    Note cytoplasmic and nuclear staining.

References

This product has been referenced in:
  • Delaunay S  et al. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. J Exp Med 213:2503-2523 (2016). Read more (PubMed: 27811057) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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