Overview

  • Product nameAnti-Allergen Jun a 3 antibody
  • Description
    Mouse polyclonal to Allergen Jun a 3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Ashe juniper (Juniperus ashei). Predicted to react with Needle juniper (Juniperus rigida) (98% identity with immunogen), Mediterranean Cypress (Cupressus sempervirens) (96% identity with immunogen), Arizona Cypress (Cupressus arizonica) (96% identity with immunogen), and Japanese cedar (Cryptomeria japonica) (94% identity with immunogen) due to sequence homology. Not yet tested in other species.
  • Immunogen

    Recombinant fusion protein: LAAGTASARF WGRTGCTFDA SGKGSCQTGD CGGQLSCTVS GAVPATLAEY TQSDQDYYDV SLVDGFNIPL AINPTNAQCT APACKADINA VCPSELKVDG , corresponding to internal sequence amino acids 61-160 of Allergen Jun a 3 from Ashe juniper (Juniperus ashei)

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 24 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • RelevanceAllergen Jun a 3 causes an allergic reaction in human.
  • Alternative names
    • Jun a3 antibody
    • Pathogenesis related protein antibody
    • Pollen allergen Jun a 3 antibody

Anti-Allergen Jun a 3 antibody images

  • All lanes : Anti-Allergen Jun a 3 antibody (ab43663) at 1/1000 dilution

    Lane 1 : Total protein extract from E.coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen
    Lane 2 : Total protein extract from E.coli with ~50ng to 500ng of the antigen fusion protein

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa

References for Anti-Allergen Jun a 3 antibody (ab43663)

ab43663 has not yet been referenced specifically in any publications.

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