The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/100 - 1/500. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended.
Acetone fixation is not recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPossible role in intracellular trafficking.
Tissue specificityExpressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).
Involvement in diseaseDefects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome.
Developmental stageWidely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
ab84892, at a 1/250 dilution, staining ALMS1 in NBF fixed asynchronous HeLa cells by Immunocytochemistry. Detection by Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100.
References for Anti-ALMS1 antibody (ab84892)
has not yet been referenced specifically in any publications.
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