Overview

  • Product name
  • Description
    Rabbit polyclonal to ALOX12B
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 423-516 (LYKLLIPHTR YTVQINSIGR AVLLNEGGLS AKGMSLGVEG FAGVMVRALS ELTYDSLYLP NDFVERGVQD LPGYYYRDDS LAVWNALEKY VTEI) of Human ALOX12B.

  • Positive control
    • Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Converts arachidonic acid to 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE).
  • Tissue specificity
    Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue.
  • Pathway
    Lipid metabolism; leukotriene D4 biosynthesis.
  • Involvement in disease
    Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE) [MIM:242100]. NCIE is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume.
  • Sequence similarities
    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12R-lipoxygenase antibody
    • 12R-LOX antibody
    • 12R-type antibody
    • Alox12b antibody
    • Arachidonate 12-lipoxygenase antibody
    • Epidermis-type lipoxygenase 12 antibody
    • LX12B_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121785 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121785, at 1/10 dilution, staining ALOX12B in paraffin-embedded Human skin tissue, by Immunohistochemistry.

References

ab121785 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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