Overview

  • Product name
  • Description
    Mouse polyclonal to ALP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human protein (AAH27870, 1 a.a. ~ 317 a.a)

  • Positive control
    • ALP transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    May play a role in the organization of actin filament arrays within muscle cells.
  • Tissue specificity
    Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actinin associated LIM protein antibody
    • Actinin-associated LIM protein antibody
    • Alpha actinin 2 associated LIM protein antibody
    • Alpha-actinin-2-associated LIM protein antibody
    • DKFZp686L0362 antibody
    • Enigma homolog antibody
    • PDLI3_HUMAN antibody
    • PDLIM 3 antibody
    • PDLIM3 antibody
    • PDZ and LIM domain 3 antibody
    • PDZ and LIM domain protein 3 antibody
    see all

Images

  • All lanes : Anti-ALP antibody (ab67228) at 1/500 dilution

    Lane 1 : ALP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 39 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Jiang S  et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016). WB ; Human . Read more (PubMed: 27432616) »
  • Wang T  et al. Osteoinduction and proliferation of bone-marrow stromal cells in three-dimensional poly (e-caprolactone)/ hydroxyapatite/collagen scaffolds. J Transl Med 13:152 (2015). WB . Read more (PubMed: 25952675) »

See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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