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Product name Anti-ALP antibody See all ALP primary antibodies
Rabbit polyclonal to ALP
Tested applications Suitable for:
WB, ELISA more details
Predicted to work with:
Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PQTVILPGPA PWGFRLSGGI DFNQPLVITR ITPGSKAAAA NLCPGDVILA) of human ALP (NP_055291).
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Purified by peptide affinity chromatography method.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Function May play a role in the organization of actin filament arrays within muscle cells.
Tissue specificity Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
Sequence similarities Contains 1 LIM zinc-binding domain. Contains 1 PDZ (DHR) domain.
Cellular localization Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
Information by UniProt
Actinin associated LIM protein antibody
Actinin-associated LIM protein antibody
Alpha actinin 2 associated LIM protein antibody
Anti-ALP antibody images
Western blot - ALP antibody (ab83259)
Anti-ALP antibody (ab83259) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 39 kDa Observed band size : 40 kDa ( why is the actual band size different from the predicted?) Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ALP antibody (ab83259)
This product has been referenced in:
et al. Association of the ACTN3 R577X polymorphism with glucose tolerance and gene expression of sarcomeric proteins in human skeletal muscle. Physiol Rep 3:N/A (2015).
Read more (PubMed: 25780092) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"