Anti-alpha + beta Tubulin antibody [TU-10] (ab30506)

Overview

  • Product nameAnti-alpha + beta Tubulin antibody [TU-10]
  • Description
    Mouse monoclonal [TU-10] to alpha + beta Tubulin
  • Specificityab30506 recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules.
  • Tested applicationsWB, ICCmore details
  • Species reactivity
    Reacts with a wide range of species. Not yet tested in other species.
  • Immunogen

    Microtubule proteins from porcine brain

  • Positive control
    • Porcine brain lysate

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by precipitation methods and size-exclusion chromatography.
  • Clonality Monoclonal
  • Clone numberTU-10
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab30506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 100 kDa. Use under reducing conditions.
ICC Use at an assay dependent dilution.

Target

  • RelevanceTubulin is the major building block of microtubules - an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides alpha and beta. Alpha- and beta-tubulins are encoded by separate genes, or small gene families. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions, the most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes and both undergo different post-translational modifications. Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
  • Cellular localizationCytoplasmic
  • Alternative names
    • B ALPHA 1 antibody
    • FLJ25113 antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • TUBA1A antibody
    • TUBA3 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2A antibody
    • Tubulin alpha 1a antibody
    • tubulin alpha 3 antibody
    • Tubulin beta 2A antibody
    • tubulin beta antibody
    see all

References for Anti-alpha + beta Tubulin antibody [TU-10] (ab30506)

ab30506 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"