The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Predicted molecular weight: 100 kDa. Use under reducing conditions.
Use at an assay dependent dilution.
RelevanceTubulin is the major building block of microtubules - an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides alpha and beta. Alpha- and beta-tubulins are encoded by separate genes, or small gene families.
Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions, the most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes and both undergo different post-translational modifications.
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin alpha 1a antibody
Tubulin beta 2A antibody
Tubulin beta antibody
Anti-alpha + beta Tubulin antibody [TU-10] images
Western blot - Anti-alpha + beta Tubulin antibody [TU-10] (ab30506)
Anti-alpha + beta Tubulin antibody [TU-10] (ab30506) at 2 µg/ml + Human Jurkat T cell line lysate (1% laurylmaltoside)
Secondary Goat anti-mouse IgG (H+L)-HRP
Performed under non-reducing conditions.
Predicted band size : 100 kDa
References for Anti-alpha + beta Tubulin antibody [TU-10] (ab30506)
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