Anti-alpha 1 Antichymotrypsin antibody (ab223226)

Overview

  • Product name
    Anti-alpha 1 Antichymotrypsin antibody
    See all alpha 1 Antichymotrypsin primary antibodies
  • Description
    Goat polyclonal to alpha 1 Antichymotrypsin
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human alpha 1 Antichymotrypsin aa 222-236 (internal sequence) (Cysteine residue). NP_001076.2.
    Sequence:

    C-DPQDTHQSRFYLSKK


    Database link: P01011

  • Positive control
    • WB: Human albumin-depleted serum and plasma lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificity
    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in disease
    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similarities
    Belongs to the serpin family.
  • Domain
    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • SERPINA3 antibody
    • AACT antibody
    • AACT_HUMAN antibody
    • ACT antibody
    • Alpha-1-antichymotrypsin His-Pro-less antibody
    • Antichymotrypsin antibody
    • Cell growth inhibiting gene 24/25 protein antibody
    • Cell growth-inhibiting gene 24/25 protein antibody
    • GIG24 antibody
    • GIG25 antibody
    • Growth inhibiting protein 24 antibody
    • Growth inhibiting protein 25 antibody
    • MGC88254 antibody
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
    • Serine proteinase inhibitor clade A member 3 antibody
    • Serpin A3 antibody
    • Serpin family A member 3 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
    • SERPINA3 antibody
    see all

Images

  • All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab223226) at 0.3 µg/ml

    Lane 1 : Human albumin-depleted serum lysate (in RIPA buffer)
    Lane 2 : Human plasma lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 65 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab223226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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