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Overview

  • Product nameAnti-alpha 1 Antichymotrypsin antibodySee all alpha 1 Antichymotrypsin primary antibodies ...
  • Description
    Sheep polyclonal to alpha 1 Antichymotrypsin
  • SpecificityThis product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity isconfirmed by double diffusion (Ouchterlony) vs human serum and a known anti-human á1 Antichymotrypsin
  • Tested applicationsDouble Immunodiffusion, Immunoelectrophoresis, RID more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Human serum α-1 anti-chymotrypsin purified from plasma.

  • General notes


    á-1 anti-chymotrypsin is a serine proteinase inhibitor with a molecular weight of 68 kDa, occuring inserum at 250-500mg/L. It appears to inhibit chymotrypsin specifically and is also a minor acute phaseprotein.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferGlycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAntiserum is prepared by immunisation of sheep with human á-1 anti-chymotrypsin and, if necessary, adsorbed tomonospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within10%. The product is 0.2µm filtered.
  • Primary antibody notes á-1 anti-chymotrypsin is a serine proteinase inhibitor with a molecular weight of 68 kDa, occuring inserum at 250-500mg/L. It appears to inhibit chymotrypsin specifically and is also a minor acute phaseprotein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Double Immunodiffusion
Immunoelectrophoresis
RID
  • Application notes
    RID: 4µL antiserum/cm2 gel vs 5µL human serum, 1/2 - 1/20
    Double diffusion: 10µL antiserum vs 5µL serum
    IEP: 100µL antiserum vs 5µL serum
    The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as Tris borate EDTA or Tris-barbital (pH ≥ 8.2)) is recommended.
    Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.

    • Target

    • FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
    • Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
    • Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
    • Sequence similaritiesBelongs to the serpin family.
    • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Cellular localizationSecreted.

      • Target information above from: UniProt accession P01011 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          SERPINA3 antibodyAACT antibodyAACT_HUMAN antibody
          ACT antibodyAlpha 1 Antichymotrypsin 1 antibodyAlpha 1 Antichymotrypsin 2 antibodyAlpha 1 Antichymotrypsin 3 antibodyAlpha 1 Antitrypsin Deficiency antibodyAlpha-1-antichymotrypsin His-Pro-less antibodyAnti Elastase antibodyAntichymotrypsin antibodyCell growth inhibiting gene 24/25 protein antibodyCell growth-inhibiting gene 24/25 protein antibodyClade A (Alpha 1 Antiproteinase Antitrypsin) antibodyGIG24 antibodyGIG25 antibodyGrowth inhibiting protein 24 antibodyGrowth inhibiting protein 25 antibodyMGC88254 antibodyPI1 antibodyProtease Inhibitor 1 antibodySerine (or Cysteine) Proteinase Inhibitor antibodySerine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibodySerine (or cysteine) proteinase inhibitor clade A member 3 antibodySerine proteinase inhibitor clade A member 3 antibodySerpin A3 antibodySerpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibodySERPINA1 antibodySERPINA2 antibodySERPINA3 antibody
        see all

      References for Anti-alpha 1 Antichymotrypsin antibody (ab8751)

      This product has been referenced in:

      See 1 Publication for this product

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      Thanks for that. Actually the method in question is Radial (not radio) immunodiffusion. Any thoughts?

      Thank you for getting back to me. In Charlotte's absence I am handling her enquiries. Unfortunately this is an antibody that is sourced externally. Charlotte has been in touch with them and unfortunately they do not have a customer protocol that they ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"