Overview

  • Product nameAnti-alpha 1 Antichymotrypsin antibody
    See all alpha 1 Antichymotrypsin primary antibodies
  • Description
    Sheep polyclonal to alpha 1 Antichymotrypsin
  • SpecificityThis product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity isconfirmed by double diffusion (Ouchterlony) vs human serum and a known anti-human á1 Antichymotrypsin
  • Tested applicationsDouble Immunodiffusion, Immunoelectrophoresis, RIDmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Human serum α-1 anti-chymotrypsin purified from plasma.

  • General notes


    á-1 anti-chymotrypsin is a serine proteinase inhibitor with a molecular weight of 68 kDa, occuring inserum at 250-500mg/L. It appears to inhibit chymotrypsin specifically and is also a minor acute phaseprotein.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferGlycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAntiserum is prepared by immunisation of sheep with human á-1 anti-chymotrypsin and, if necessary, adsorbed tomonospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within10%. The product is 0.2µm filtered.
  • Primary antibody notes á-1 anti-chymotrypsin is a serine proteinase inhibitor with a molecular weight of 68 kDa, occuring inserum at 250-500mg/L. It appears to inhibit chymotrypsin specifically and is also a minor acute phaseprotein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Double Immunodiffusion
Immunoelectrophoresis
RID
  • Application notes
    RID: 4µL antiserum/cm2 gel vs 5µL human serum, 1/2 - 1/20
    Double diffusion: 10µL antiserum vs 5µL serum
    IEP: 100µL antiserum vs 5µL serum
    The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as Tris borate EDTA or Tris-barbital (pH ≥ 8.2)) is recommended.
    Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
  • Target

    • FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
    • Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
    • Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
    • Sequence similaritiesBelongs to the serpin family.
    • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • SERPINA3 antibody
      • AACT antibody
      • AACT_HUMAN antibody
      • ACT antibody
      • Alpha 1 Antichymotrypsin 1 antibody
      • Alpha 1 Antichymotrypsin 2 antibody
      • Alpha 1 Antichymotrypsin 3 antibody
      • Alpha 1 Antitrypsin Deficiency antibody
      • Alpha-1-antichymotrypsin His-Pro-less antibody
      • Anti Elastase antibody
      • Antichymotrypsin antibody
      • Cell growth inhibiting gene 24/25 protein antibody
      • Cell growth-inhibiting gene 24/25 protein antibody
      • Clade A (Alpha 1 Antiproteinase Antitrypsin) antibody
      • GIG24 antibody
      • GIG25 antibody
      • Growth inhibiting protein 24 antibody
      • Growth inhibiting protein 25 antibody
      • MGC88254 antibody
      • PI1 antibody
      • Protease Inhibitor 1 antibody
      • Serine (or Cysteine) Proteinase Inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
      • Serine proteinase inhibitor clade A member 3 antibody
      • Serpin A3 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
      • SERPINA1 antibody
      • SERPINA2 antibody
      • SERPINA3 antibody
      see all

    References for Anti-alpha 1 Antichymotrypsin antibody (ab8751)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Thank you for getting back to me. In Charlotte's absence I am handling her enquiries. Unfortunately this is an antibody that is sourced externally. Charlotte has been in touch with them and unfortunately they do not have a customer protocol that th...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"