alpha 1 Antitrypsin Human ELISA Kit (ab108798)
- Product namealpha 1 Antitrypsin Human ELISA KitSee all alpha 1 Antitrypsin kits ...
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.04 µg/ml
- Range0.039 µg/ml - 40 µg/ml
- Assay time3h 00m
- Species reactivityReacts with: Human
- Product overview
Alpha 1 antitrypsin (A1AT) is a protein that protects the lungs. The liver usually makes the protein, and releases it into the bloodstream. alpha 1 Antitrypsin is a major protease inhibitor that controls tissue degradation. A reduction of alpha 1 Antitrypsin levels can cause a change in collagen metabolism. alpha 1 Antitrypsin inhibits neutrophil elastase release into the lungs during inflammatory states. alpha 1 Antitrypsin deficiency is an uncommon genetic disease that can lead to emphysema, hepatitis, cirrhosis, chronic obstructive pulmonary disease (COPD).
ab108798 alpha 1 Antitrypsin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human alpha 1 Antitrypsin in plasma, and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human alpha 1 Antitrypsin in less than 3 hours. A polyclonal antibody specific for human alpha 1 Antitrypsin has been pre-coated onto a 96-well microplate with removable strips. alpha 1 Antitrypsin in standards and samples is competed by a biotinylated alpha 1 Antitrypsin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated alpha 1 Antitrypsin 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human alpha 1 Antitrypsin Microplate 1 x 96 tests Human alpha 1 Antitrypsin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
- AATAlpha 1 antiproteinaseAlpha 1 protease inhibitorAlpha-1 protease inhibitorAlpha-1-antiproteinasealpha-1-antitrypsinalpha-1-antitrypsin nullalpha1ATPIPI1PRO0684PRO2275protease inhibitor 1 (anti-elastase)Serine (or cysteine) proteinase inhibitor clade A member 1serine (or cysteine) proteinase inhibitor, clade A, member 1Serpin A1Serpin peptidase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 1Serpina1Short peptide from AATSPAAT
Our Abpromise guarantee covers the use of ab108798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for alpha 1 Antitrypsin Human ELISA Kit (ab108798)
ab108798 has not yet been referenced specifically in any publications.