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alpha 1 Antitrypsin Human ELISA Kit (ab108798)

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Overview

  • Product namealpha 1 Antitrypsin Human ELISA KitSee all alpha 1 Antitrypsin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.04 µg/ml
  • Range
    0.039 µg/ml - 40 µg/ml
  • Recovery

    98 %

  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Alpha 1 antitrypsin (A1AT) is a protein that protects the lungs. The liver usually makes the protein, and releases it into the bloodstream. alpha 1 Antitrypsin is a major protease inhibitor that controls tissue degradation. A reduction of alpha 1 Antitrypsin levels can cause a change in collagen metabolism. alpha 1 Antitrypsin inhibits neutrophil elastase release into the lungs during inflammatory states. alpha 1 Antitrypsin deficiency is an uncommon genetic disease that can lead to emphysema, hepatitis, cirrhosis, chronic obstructive pulmonary disease (COPD).

    ab108798 alpha 1 Antitrypsin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human alpha 1 Antitrypsin in plasma, and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human alpha 1 Antitrypsin in less than 3 hours. A polyclonal antibody specific for human alpha 1 Antitrypsin has been pre-coated onto a 96-well microplate with removable strips. alpha 1 Antitrypsin in standards and samples is competed by a biotinylated alpha 1 Antitrypsin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsCompetitive ELISA more details

Properties

  • FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD) [MIM:613490]. A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age.
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localizationSecreted > extracellular space > extracellular matrix and Secreted.

    • Target information above from: UniProt accession P01009 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        A1AA1ATA1AT_HUMAN
        AATAlpha 1 antiproteinaseAlpha 1 protease inhibitorAlpha-1 protease inhibitorAlpha-1-antiproteinasealpha-1-antitrypsinalpha-1-antitrypsin nullalpha1ATPIPI1PRO0684PRO2275protease inhibitor 1 (anti-elastase)Serine (or cysteine) proteinase inhibitor clade A member 1serine (or cysteine) proteinase inhibitor, clade A, member 1Serpin A1Serpin peptidase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 1Serpina1Short peptide from AATSPAAT
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108798 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Competitive ELISA cELISA

      alpha 1 Antitrypsin Human ELISA Kit images

      • ELISA - alpha 1 Antitrypsin Human ELISA Kit 1x96 (ab108798)
        ELISA - alpha 1 Antitrypsin Human ELISA Kit 1x96 (ab108798)

      Protocols

      References for alpha 1 Antitrypsin Human ELISA Kit (ab108798)

      ab108798 has not yet been referenced specifically in any publications.

      Publishing research using ab108798 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108798 Submit a Question for ab108798

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      Product code: 108798
      Inquiry: Hi, I wondered if you could help. I am looking for an ELISA kit for human alpha-1-antitrypsin. I will be using serum and sputum samples. I noticed you have two A1AT ELISA kits - ab108798 and ab108799. Do you know if eithe...

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      Thank you for contacting us.

      We unfortunately have not tested these products with sputum samples. Theoretically, these products will be OK however we cannot guarantee as we do not have tested data. The best way for using these products is by incl...

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      Following the email confirmation below from our customer, they have decided to get the replacement kit to be tested.

      Can you arrange for the similar replacement kit to be send out as soon as possible?

      We look forward to your soo...

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      Thank you for your email.

      I have added now a free unit of this kit ab108798 to your next order with the reference number xx. It will be dispatched tomorrow.

      I can also confirm, that I have been in correspondence with the laboratory, th...

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      Dear Technical,

      Our customer confirmed to have centrifuge the reagent prior to use & they received only 15uL of reagent.

      Looking forward to your soonest reply.

      Best regards,

      This is to confirm that we are liaising with the lab to get this specific kit component ready for shipment as soon as possible. Thank you for your patience in advance!

      Dear Technical,

      Our customer confirms that there was shortage of streptavidin conjugate reagent. Can you please arrange for additional reagent to be sent immediately?

      We look forward to your soonest reply.



      Than...

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      Thank you for your reply.

      Please could you confirm the following:

      1. Did the customer centrifuge the component?
      2. How much did they receive?

      Looking forward to your reply.

      Hi Technical,

      Our customer had purchased 1 kit alpha- 1-Antitrypsin Human ELISA Kit (ab108798) recently and complained that the streptavidin conjugate reagent is insufficient for 96 wells plate. Other ordering details are as follows:

      Read More

      Thank you for your enquiry regarding ab108798.

      Could you please confirm and explain why the streptavidin conjugate reagent is insufficient for 96 wells plate? Is there any shortage in the volume provided or the reagent does not work properly? Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"