Overview

  • Product nameAnti-alpha 1 Antitrypsin antibody [B9]See all alpha 1 Antitrypsin primary antibodies ...
  • Description
    Mouse monoclonal [B9] to alpha 1 Antitrypsin
  • SpecificityRecognises alpha 1-antitrypsin. Does not react with other human plasma proteins.
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human protein

Properties

Applications

Our Abpromise guarantee covers the use of ab9399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificityPlasma.
  • Involvement in diseaseAlpha-1-antitrypsin deficiency
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localizationSecreted > extracellular space > extracellular matrix and Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha-1-AT antibody
    • alpha1 PI antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Clade A (alpha 1 antiproteinase antitrypsin) member 1 antibody
    • Dom1 antibody
    • MGC23330 antibody
    • MGC9222 antibody
    • PI antibody
    • PI1 antibody
    • PRO2275 antibody
    • Protease inhibitor 1 (anti elastase) antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 1 antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • SerpinA1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Anti-alpha 1 Antitrypsin antibody [B9] images

  • Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5 µg/ml.
    lane 2 - human plasma, 2 µl
    lane S - standard proteins.
    A - SDS-PAGE, Coomasie staining
    B - Western blot with ab9399.

  • Ab9399 staining human normal liver. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/ml + Active human alpha 1 Antitrypsin full length protein (ab91136) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

This product has been referenced in:
  • Chen Y  et al. Role of malectin in Glc(2)Man(9)GlcNAc(2)-dependent quality control of a1-antitrypsin. Mol Biol Cell 22:3559-70 (2011). WB ; Human . Read more (PubMed: 21813736) »
  • Liu Z  et al. Tandem 18O stable isotope labeling for quantification of N-glycoproteome. J Proteome Res 9:227-36 (2010). WB ; Human . Read more (PubMed: 19921957) »

See all 7 Publications for this product

Product Wall

Thank you for your reply, this information is very helpful. Please let me know if you need any further assistance with any of our products.

Thank you for contacting us.
While we have validated ab9399 and ab7634 for use in ELISA, this has been done in indirect and direct ELISA respectively, where an antigen was coated to the plate and these antibodies were used separately for detection...

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Thank you for your inquiry. The alpha 1 Antitrypsin (AAT) antibody ab9399 is raised against the full-length human protein. However, the epitope has not been mapped and it is therefore not possible to identify the sequence which is recognised by...

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Thank you for your enquiry. Unfortunately we do not have a pair of antibodies that have been shown to work as a pair in sandwich ELISA. All of our antibodies are tested and guaranteed to work by direct ELISA against the antigen that they were raise...

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Thank you for your enquiry. To our knowledge this antibody has yet to be tested in this application, therefore we are not certain if it will work in IHC or IF. If you choose to purchase this antibody for testing in applications for which we don't have ...

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This antibody has not been tested in drosophila. This antibody has been raised against full-length protein. The epitope recognized by this antibody is not determined. We do not routinely offer free or trial sized samples for testing purposes. Our p...

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Thank you for your email. Abcam does not have a custom conjugation service. If I can be of additional assistance, please contact me again.

Thank you for your email. The molecular weight of alpha 1 Antitrypsin is 52 kDa. If you have any more questions, please let us know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"