Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)
- Product nameAnti-alpha 1 Antitrypsin antibody [B9]See all alpha 1 Antitrypsin primary antibodies ...
- DescriptionMouse monoclonal [B9] to alpha 1 Antitrypsin
- SpecificityRecognises alpha 1-antitrypsin. Does not react with other human plasma proteins.
- Tested applicationsELISA, WB, IHC-P more details
- Species reactivityReacts with: Human
Full length human protein
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 0.1% sodium azide
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone numberB9
- Light chain typeunknown
- Research Areas
Our Abpromise guarantee covers the use of ab9399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||ELISA: Use at an assay dependent concentration.|
|WB||WB: Use at an assay dependent concentration.|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
- FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
- Tissue specificityPlasma.
- Involvement in diseaseAlpha-1-antitrypsin deficiency (A1ATD) [MIM:613490]: A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the serpin family.
- DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
- Cellular localizationSecreted > extracellular space > extracellular matrix and Secreted.
- A1A antibodyA1AT antibodyA1AT_HUMAN antibody
- AAT antibodyAlpha 1 antiproteinase antibodyAlpha 1 antitrypsin antibodyAlpha 1 antitrypsin null antibodyAlpha 1 protease inhibitor antibodyAlpha-1 protease inhibitor antibodyAlpha-1-antiproteinase antibodyalpha-1-AT antibodyalpha1 PI antibodyalpha1 proteinase inhibitor antibodyAlpha1AT antibodyClade A (alpha 1 antiproteinase antitrypsin) member 1 antibodyDom1 antibodyMGC23330 antibodyMGC9222 antibodyPI antibodyPI1 antibodyPRO2275 antibodyProtease inhibitor 1 (anti elastase) antibodySerine (or cysteine) proteinase inhibitor clade A member 1 antibodySerine protease inhibitor 1-1 antibodySerine protease inhibitor A1a antibodySerpin A1 antibodySerpin A1a antibodySerpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 1 antibodySerpin peptidase inhibitor clade A member 1 antibodySerpinA1 antibodyShort peptide from AAT antibodySPAAT antibodySpi1-1 antibody
Anti-alpha 1 Antitrypsin antibody [B9] images
Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5
lane 2 - human plasma, 2
lane S - standard proteins.
A - SDS-PAGE, Coomasie staining
B - Western blot with ab9399.
Ab9399 staining human normal liver. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/ml +
alpha 1 Antitrypsin protein (Active) (ab91136) at 0.1 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)
This product has been referenced in:
- Chen Y et al. Role of malectin in Glc(2)Man(9)GlcNAc(2)-dependent quality control of a1-antitrypsin. Mol Biol Cell 22:3559-70 (2011). WB ; Human . Read more (PubMed: 21813736) »
- Liu Z et al. Tandem 18O stable isotope labeling for quantification of N-glycoproteome. J Proteome Res 9:227-36 (2010). WB ; Human . Read more (PubMed: 19921957) »