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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Overview
Product name
Anti-alpha 1 Catenin antibody
See all alpha 1 Catenin products (11) ...
Description
Rabbit polyclonal to alpha 1 Catenin
Specificity
Anti-alpha-E-Catenin recognizes alpha-E-catenin (102 kDa). Staining of the alpha-E-catenin band in immunoblotting is specifically inhibited with the alpha-E-catenin immunizing peptide (human, amino acids 873-887).
Tested applications
IHC-Fr, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Xenopus laevis
Immunogen
Synthetic peptide: EKQDETQTKIKRASQ conjugated to KLH, corresponding to amino acids 873-887 of Human alpha 1 Catenin.
EKQDETQTKI KRASQ
Positive control
WB: whole cell extract of human epidermal carcinoma A431 cell line, cytosolic fraction S1 of rat embryonic brain. ICC: MDCK or MCF cells
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Catenins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/500IHC-Fr: 1/500
ICC/IF: 1/100(Fix with methanol.)
WB: 1/1000Detects a band of approximately 102 kDa.
Target
Relevance
The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.
Cellular localization
Cell Membrane and Cytoplasmic
Alternative names
- alpha catenin antibody
- Alpha E Catenin antibody
- alphaE catenin antibody
see all
Database links
- Entrez Gene: 1495 Human
- Entrez Gene: 12385 Mouse
- Entrez Gene: 399303 Xenopus laevis
- Omim: 116805 Human
- SwissProt: P35221 Human
- SwissProt: P26231 Mouse
- Unigene: 534797 Human
References for Anti-alpha 1 Catenin antibody (ab11346)
This product has been referenced in:
- Thompson Jet al. Adherens junction breakdown in the periderm following cadmium administration in the chick embryo: distribution of cadherins and associated molecules. Reprod Toxicol 25:39-46 (2008).Read more (PubMed: 18031986) »
- Stocker AM & Chenn A Differential expression of alpha-E-catenin and alpha-N-catenin in the developing cerebral cortex. Brain Res 1073-1074:151-8 (2006).Read more (PubMed: 16457793) »
See all 2 publications for this product
Publishing research using ab11346? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"