Anti-alpha 1 Catenin antibody (ab52227)

Overview

  • Product nameAnti-alpha 1 Catenin antibodySee all alpha 1 Catenin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha 1 Catenin
  • Specificityab52227 detects endogenous levels of total alpha 1 Catenin protein.
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human alpha 1 Catenin.

  • Positive control
    • HeLa cells extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab52227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ELISA 1/10000.

Target

  • RelevanceThe distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.
  • Cellular localizationCell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • alpha catenin antibody
    • alpha E catenin antibody
    • alphaE catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • CTNNA 1 antibody
    • CTNNA1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    see all

Anti-alpha 1 Catenin antibody images

  • All lanes : Anti-alpha 1 Catenin antibody (ab52227) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell Lysate; ab52227 pre-incubated with immunising (blocking) peptide


    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • ab52227 (1/100) staining alpha 1 Catenin in paraffin-embedded human breast carcinoma tissue. Right hand panel represents a negative control for which ab52227 was pre-incubated with the immunising peptide (blocking).
  • Western blot of ab52227 detecting alpha 1 catenin protein. Mouse small intestine epithelial cells were isolated and run on a 9% denaturing gel. The gel was blocked with 5% milk for 1 hour before being incubated with ab52227 (1:1000) for 12 hours at 4°C in 5% Milk/TBS Tween. The antibody was detected using anti-rabbit IgG conjugated to HRP (1:2500).  Lane 1 is an intestinal epithelial lysate taken from a conditional Adenomatous Polyposis Coli (APC) mutant mouse, while lane 2 was taken from a control.

References for Anti-alpha 1 Catenin antibody (ab52227)

ab52227 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Small Intestine Epithelial cells)
Loading amount 7.5 µg
Specification Small Intestine Epithelial cells
Gel Running Conditions Reduced Denaturing (9% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 12 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"