Anti-alpha 1 Catenin antibody (ab52227)

Overview

  • Product nameAnti-alpha 1 Catenin antibodySee all alpha 1 Catenin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha 1 Catenin
  • Specificityab52227 detects endogenous levels of total alpha 1 Catenin protein.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human alpha 1 Catenin.

  • Positive control
    • HeLa cells extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab52227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ELISA 1/10000.

Target

  • FunctionAssociates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificityExpressed ubiquitously in normal tissues.
  • Sequence similaritiesBelongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha catenin antibody
    • alpha E catenin antibody
    • Alpha E-catenin antibody
    • alphaE catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • CTNNA1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Anti-alpha 1 Catenin antibody images

  • All lanes : Anti-alpha 1 Catenin antibody (ab52227) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell Lysate; ab52227 pre-incubated with immunising (blocking) peptide


    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • ab52227 (1/100) staining alpha 1 Catenin in paraffin-embedded human breast carcinoma tissue. Right hand panel represents a negative control for which ab52227 was pre-incubated with the immunising peptide (blocking).
  • Western blot of ab52227 detecting alpha 1 catenin protein. Mouse small intestine epithelial cells were isolated and run on a 9% denaturing gel. The gel was blocked with 5% milk for 1 hour before being incubated with ab52227 (1:1000) for 12 hours at 4°C in 5% Milk/TBS Tween. The antibody was detected using anti-rabbit IgG conjugated to HRP (1:2500).  Lane 1 is an intestinal epithelial lysate taken from a conditional Adenomatous Polyposis Coli (APC) mutant mouse, while lane 2 was taken from a control.

References for Anti-alpha 1 Catenin antibody (ab52227)

ab52227 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Small Intestine Epithelial cells)
Loading amount 7.5 µg
Specification Small Intestine Epithelial cells
Gel Running Conditions Reduced Denaturing (9% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 12 2008

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