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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Overview
Product name
Anti-alpha 1 Catenin antibody
See all alpha 1 Catenin products (11) ...
Description
Rabbit polyclonal to alpha 1 Catenin
Specificity
ab52227 detects endogenous levels of total alpha 1 Catenin protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from human alpha 1 Catenin.
Positive control
HeLa cells extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Catenins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Relevance
The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.
Cellular localization
Cell Membrane and Cytoplasmic
Alternative names
- alpha catenin antibody
- alpha E catenin antibody
- alphaE catenin antibody
see all
Database links
- Entrez Gene: 1495 Human
- Entrez Gene: 12385 Mouse
- Entrez Gene: 307505 Rat
- Omim: 116805 Human
- SwissProt: P35221 Human
- SwissProt: P26231 Mouse
- Unigene: 534797 Human
Anti-alpha 1 Catenin antibody images:
Western blot - alpha 1 Catenin antibody (ab52227)
All lanes : Anti-alpha 1 Catenin antibody (ab52227) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell Lysate; ab52227 pre-incubated with immunising (blocking) peptide
Predicted band size : 100 kDa
Observed band size : 100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 1 Catenin antibody (ab52227)
ab52227 (1/100) staining alpha 1 Catenin in paraffin-embedded human breast carcinoma tissue. Right hand panel represents a negative control for which ab52227 was pre-incubated with the immunising peptide (blocking).
- alpha 1 Catenin antibody (ab52227)
Western blot of ab52227 detecting alpha 1 catenin protein. Mouse small intestine epithelial cells were isolated and run on a 9% denaturing gel. The gel was blocked with 5% milk for 1 hour before being incubated with ab52227 (1:1000) for 12 hours at 4°C in 5% Milk/TBS Tween. The antibody was detected using anti-rabbit IgG conjugated to HRP (1:2500). Lane 1 is an intestinal epithelial lysate taken from a conditional Adenomatous Polyposis Coli (APC) mutant mouse, while lane 2 was taken from a control.
References for Anti-alpha 1 Catenin antibody (ab52227)
ab52227 has not yet been referenced specifically in any publications.
Publishing research using ab52227? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"