You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab10071 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
 | |||||||||
|
|||||||||
At this point, I don’t want to ask more from you unless I know it will work. Use your antibody as capture, if I go with anti-goat AFP as detector, it works. It’s just that the anti-Rabbit detector somehow doesn’t work. Thank you again for your patience to me! |
|||||||||
ANSWER: |
Thanks for your reply, and I am sorry that I don't have a better explanation for this problem. If there is anything else that we can do for you, please let me know and I'll be happy to help. |
||||||||
|
|||||||||
Thanks a lot for your help!I’ll try it again. I’ll let you know if it works.Have a great weekend! |
|||||||||
ANSWER: |
I just wanted to thank you for completing our follow-up survey recently, and I'm sorry to see that this problem persisted even with the replacement vial of antibody. Since the results are still not satisfactory, I would be happy to issue a credit or refund for your purchase, or I can send a new vial of your choosing. Please let me know which you would prefer, or if there is anything else that we can do for you. |
||||||||
|
|||||||||
I purchased antibodies for detecting AFP in serum by ELISA assay. Details of my antibodies are: 1. alpha 1 Fetoprotein antibody (ab 10071) with lot number #156917 2. alpha 1 fetoprotein antibody (ab 10072) with lot number #159812 Those antibodies will be applied as capture (no 1) and detecting (no 2) antibodies. What I want to know is what dilution should I perform for those antibodies to optimize my ELISA assay in measuring AFP level in serum? Thanks for your help |
|||||||||
ANSWER: |
Thank you for your enquiry. Unless a dilution is listed on the data sheet, we do not know the dilution to use for sandwich ELISA. A few things to keep in mind are that typically you do not need a lot of antibody to do an ELISA, just enough to coat the wells. Perhaps you could start with 0.1 ug/mL. ELISAs can be tricky because there is a bit of trial and error involved if you don't have a recommended dilution. It may take some time to optimize your assay. Maybe you could try a few dilutions between 0.1 ug/ml and 5 ug/ml and see how that goes. I am sorry that I do not have more information. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
||||||||
|
|||||||||
| |||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"