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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-alpha 1 Fetoprotein antibody [4A3]
See all alpha 1 Fetoprotein products (31) ...
Description
Mouse monoclonal [4A3] to alpha 1 Fetoprotein
Specificity
ab10071 and 10072 react with different determinants of human a-fetoprotein. They do not react with albumin.
Tested applications
Sandwich ELISA, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (purified) (Human).
General notes
Concentration varies from lot to lot and can be provided on request.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Purity tested by electrophoresis.
Clonality
Monoclonal
Clone number
4A3
Myeloma
Sp2/0
Isotype
IgG2a
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cancer >> Tumor biomarkers >> Tumor antigens
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5H7] to alpha 1 Fetoprotein (HRP) (ab10072). (Can be used as capture antibody.)
ELISA: Use at an assay dependent dilution.
Target
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac.
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational
modifications
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFP antibody
- alpha 1 fetoprotein antibody
- alpha fetoglobulin antibody
see all
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
References for Anti-alpha 1 Fetoprotein antibody [4A3] (ab10071)
This product has been referenced in:
- Schmelzer Eet al. Three-dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells. Tissue Eng Part A 16:2007-16 (2010). Sandwich ELISA; Human.Read more (PubMed: 20088704) »
See 1 publication for this product
Publishing research using ab10071? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"