![Immunocytochemistry/ Immunofluorescence - alpha 1 Fetoprotein antibody [AFP-01] (ab3980) image](#alpha-1-Fetoprotein-Primary-antibodies-ab3980-1.jpg)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-alpha 1 Fetoprotein antibody [AFP-01]
See all alpha 1 Fetoprotein products (31) ...
Description
Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein
Specificity
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Tested applications
Sandwich ELISA, ICC/IF, ELISA, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified) (Human).
Positive control
Hep G2 human hepatocellular carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by precipitation methods.
Clonality
Monoclonal
Clone number
AFP-01
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cancer >> Tumor biomarkers >> Tumor antigens
Cardiovascular >> Blood >> Serum Proteins
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Immunocytochemistry/ Immunofluorescence - alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
![Immunocytochemistry/ Immunofluorescence - alpha 1 Fetoprotein antibody [AFP-01] (ab3980) image](/ps/datasheet/images/3/ab3980/alpha-1-Fetoprotein-Primary-antibodies-ab3980-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [AFP-11] to alpha 1 Fetoprotein (ab3969). (Can be used as detection antibody.)
ICC/IF: Use a concentration of 5 µg/ml
ELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [AFP-11] to alpha 1 Fetoprotein (ab3969).
IP: Use at an assay dependent dilution. (Use at an assay dependant dilution. Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.)
WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa.
Target
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac.
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational
modifications
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFP antibody
- Alpha fetoglobulin antibody
- Alpha fetoprotein antibody
see all
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
Anti-alpha 1 Fetoprotein antibody [AFP-01] images:
Immunocytochemistry/ Immunofluorescence - alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
This product has been referenced in:
- Colquhoun DRet al. Global screening of human cord blood proteomes for biomarkers of toxic exposure and effect. Environ Health Perspect 117:832-8 (2009). WB; Human.Read more (PubMed: 19478969) »
- Colquhoun DRet al. Global screening of human cord blood proteomes for biomarkers of toxic exposure and effect. Environ Health Perspect 117:832-8 (2009). WB; Human.Read more (PubMed: 19478969) »
See all 4 publications for this product
Publishing research using ab3980? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"