alpha 1 Fetoprotein antibody [AFP-01] (ab3980)

Product Name 

alpha 1 Fetoprotein antibody [AFP-01]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein

Immunogen 

Full length native protein (purified) (Human).

Reacts with 
(species key)

Hu

Specificity 

The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

Tested applications 
(see key)

ELISA, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
ELISA: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution. Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents).
Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
WB: Use at an assay dependant dilution. Detects a band of approximately 70 kDa. Use under non-reducing or reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hep G2 human hepatocellular carcinoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.

Cellular localization 

Secreted

Research areas 

Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cancer >> Tumor biomarkers >> Tumor antigens
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AFP-01

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites by precipitation methods.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for alpha 1 Fetoprotein antibody [AFP-01] images, references, products related to ab3980 and other tools.

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References for alpha 1 Fetoprotein antibody [AFP-01] (ab3980)

  This product has been used in: (two most recent references)

Cavin LG et al. Regulation of alpha-fetoprotein by nuclear factor-kappaB protects hepatocytes from tumor necrosis factor-alpha cytotoxicity during fetal liver development and hepatic oncogenesis. Cancer Res 64:7030-8 (2004). PubMed: 15466196

Stefanova I et al. Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods 111:67-73 (1988). PubMed: 2455753
If you publish research using ab3980 please let us know so that we can cite the reference on this datasheet.

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