Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-alpha 1 Fetoprotein antibody
See all alpha 1 Fetoprotein products (31) ...
Description
Mouse monoclonal to alpha 1 Fetoprotein
Tested applications
WB, IHC-P, Sandwich ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 500-610 of Human alpha 1 Fetoprotein
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG
Light chain type
kappa
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cancer >> Tumor biomarkers >> Tumor antigens
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P: Use a concentration of 3 µg/ml.
sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to alpha 1 Fetoprotein (ab8201). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection.
ICC/IF: Use a concentration of 10 µg/ml.
Target
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac.
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational
modifications
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFP antibody
- Alpha fetoglobulin antibody
- Alpha fetoprotein antibody
see all
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
Anti-alpha 1 Fetoprotein antibody images:
IHC-P - alpha 1 Fetoprotein antibody (ab54745)
alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
Western blot - alpha 1 Fetoprotein antibody (ab54745)
alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence - alpha 1 Fetoprotein antibody (ab54745)
ab54745 at 10 ug/ml staining alpha Fetoprotein in human Hela cells by Immunocytochemistry / Immunofluorescence.
Sandwich ELISA - alpha 1 Fetoprotein antibody (ab54745)
Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml
Protocols
References for Anti-alpha 1 Fetoprotein antibody (ab54745)
ab54745 has not yet been referenced specifically in any publications.
Publishing research using ab54745? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"