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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-alpha 1 Fetoprotein antibody
See all alpha 1 Fetoprotein products (31) ...
Description
Rabbit polyclonal to alpha 1 Fetoprotein
Specificity
Specificity of the antisera is confirmed by ELISA (OPD) on human alpha-Fetoprotein versus albumin, IgG, IgM. Ouchterlony double diffusion, immunoelectrophoresis. No crossreactivity with human plasma proteins.
Tested applications
Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: None
Constituents: Mannitol, Dextran, salts.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antiserum was subjected to absorption with immobilized human albumin fraction and globulin fractions and fibrinogen in order to remove non-specific antibodies. The affinity purified antibody is obtained by binding to immobilized human alpha-Fetoprotein (antigen used for immunization), followed by elution with acidic buffer, titration, dialysis, dispensing and lyophilization.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cancer >> Tumor biomarkers >> Tumor antigens
Cardiovascular >> Blood >> Serum Proteins
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Sandwich ELISA - alpha 1 Fetoprotein antibody (ab8201)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab8201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.1 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal to alpha 1 Fetoprotein (ab54745). (For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab54745 as Capture.)
AP: Use at an assay dependent concentration.
Target
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac.
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational
modifications
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFP antibody
- Alpha fetoglobulin antibody
- Alpha fetoprotein antibody
see all
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
Anti-alpha 1 Fetoprotein antibody images:
Sandwich ELISA - alpha 1 Fetoprotein antibody (ab8201)
Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml
Protocols
References for Anti-alpha 1 Fetoprotein antibody (ab8201)
ab8201 has not yet been referenced specifically in any publications.
Publishing research using ab8201? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"