alpha 1 Microglobulin Human ELISA Kit (ab108884)
- Product namealpha 1 Microglobulin Human ELISA KitSee all alpha 1 Microglobulin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.6 ng/ml
- Range0.625 ng/ml - 40 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Alpha 1 microglobulin, also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfamily for small hydrophobic molecules. It contains 184 amino acids and weighs 26-kDa. Mature alpha 1 microglobulin and bikunin (urinary trypsin inhibitor) result from a common precursor. Alpha 1 microglobulin is found in blood both in free form and complex-bound to immunoglobulin A (IgA). It is involved in inflammatory and detoxification processes caused by immune system activation, and extracellular heme catabolism. While increased excretion was detected in urine or serum shortly after tubular injury, alpha 1 microglobulin may predict acute kidney injury and the need for renal replacement therapy. Urinary alpha 1 microglobulin is useful for the early detection of nephropathy in type 2 diabetic subjects.
ab108884 is designed for detection of human alpha 1 microglobulin in plasma, serum, urine, saliva, milk, and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human alpha 1 microglobulin in less than 4 hours. A polyclonal antibody specific for human alpha 1 microglobulin has been pre-coated onto a 96-well microplate with removable strips. Alpha 1 microglobulin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human alpha 1 microglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated alpha 1 Microglobulin Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human alpha 1 Microglobulin Microplate 1 unit Human alpha 1 Microglobulin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- SwissProt: P02760 Human
Our Abpromise guarantee covers the use of ab108884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for alpha 1 Microglobulin Human ELISA Kit (ab108884)
ab108884 has not yet been referenced specifically in any publications.