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alpha 1 Microglobulin Human ELISA Kit (ab108884)

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Overview

  • Product namealpha 1 Microglobulin Human ELISA KitSee all alpha 1 Microglobulin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.6 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    96 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Alpha 1 microglobulin, also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfamily for small hydrophobic molecules. It contains 184 amino acids and weighs 26-kDa. Mature alpha 1 microglobulin and bikunin (urinary trypsin inhibitor) result from a common precursor. Alpha 1 microglobulin is found in blood both in free form and complex-bound to immunoglobulin A (IgA). It is involved in inflammatory and detoxification processes caused by immune system activation, and extracellular heme catabolism. While increased excretion was detected in urine or serum shortly after tubular injury, alpha 1 microglobulin may predict acute kidney injury and the need for renal replacement therapy. Urinary alpha 1 microglobulin is useful for the early detection of nephropathy in type 2 diabetic subjects.

    ab108884 is designed for detection of human alpha 1 microglobulin in plasma, serum, urine, saliva, milk, and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human alpha 1 microglobulin in less than 4 hours. A polyclonal antibody specific for human alpha 1 microglobulin has been pre-coated onto a 96-well microplate with removable strips. Alpha 1 microglobulin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human alpha 1 microglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108884 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      alpha 1 Microglobulin Human ELISA Kit images

      • Sandwich ELISA - alpha 1 Microglobulin Human ELISA Kit - 1 x 96 Wells Plate (ab108884)
        Sandwich ELISA - alpha 1 Microglobulin Human ELISA Kit - 1 x 96 Wells Plate (ab108884)

      Protocols

      References for alpha 1 Microglobulin Human ELISA Kit (ab108884)

      ab108884 has not yet been referenced specifically in any publications.

      Publishing research using ab108884 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108884 Submit a Question for ab108884

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      My collaborator bought some months ago different ELISA kits from your compagny. To understand our results, we need to known the sequence which is recognised by the antibodies used in these. Please can you send me this information for the following referen...

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      Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provide ...

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      To refine my choice, I would like to know against which protein(s) (sequence or accesion number in Swissprot) are exactly directed antibodies used in the following Elisa kits:   ab108884 ab108883 ab108823 ab108841   Thanks in advance      

      Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfamily ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"