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Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)

Abreviews (24)Q&A (39)Specific References (36)

Overview

  • Product nameAnti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane MarkerSee all alpha 1 Sodium Potassium ATPase primary antibodies ...
  • Description
    Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase - Plasma Membrane Marker
  • SpecificityThis antibody is specific for Na,K-ATPase alpha 1 subunit
  • Tested applicationsIHC-P, ICC/IF, WB, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Full length native protein (purified) (Rabbit renal outermedulla).

  • Positive controlThis antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
  • General notesThis product is available conjugated to FITC. Please see ab150007. This antibody has been used as a plasma membrane marker.

Properties

Applications

Our Abpromise guarantee covers the use of ab7671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use a concentration of 5 µg/ml.
ICC/IF ICC/IF: Use a concentration of 5 - 10 µg/ml.
WB WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
Flow Cyt Flow Cyt: Use a concentration of 10 µg/ml. See Abreview.

Target

  • Alternative names
      AT1A1_HUMAN antibodyATP1A1 antibodyAtpa-1 antibody
      ATPase Na+/K+ transporting alpha 1 polypeptide antibodyBC010319 antibodyEC 3.6.3.9 antibodyMGC1798 antibodyMGC25056 antibodyMGC3285 antibodyMGC38419 antibodyMGC51750 antibodyNa K ATPase alpha A catalytic polypeptide antibodyNa K ATPase catalytic subunit alpha A protein antibodyNa(+)/K(+) ATPase 1 antibodyNa(+)/K(+) ATPase alpha-1 subunit antibodyNa+/K+ ATPase alpha 1 subunit antibodyNa+/K+ ATPase 1 antibodyNkaa1b antibodySodium potassium ATPase alpha 1 polypeptide antibodySodium pump 1 antibodySodium pump subunit alpha-1 antibodySodium/potassium dependent ATPase beta 1 subunit antibodySodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker images

  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Lanes 1 - 3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1 µg/ml
    Lanes 4 - 6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 2 µg/ml
    Lanes 7 - 9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 5 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 6 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 8 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 9 : Brain (Rat) Tissue Lysate (ab7942)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa


    Exposure time : 3 minutes
  • Western blot - alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Western blot - alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
    Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
    Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Western blot - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Western blot - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate

    Predicted band size : 112 kDa
  • Immunocytochemistry/ Immunofluorescence - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Immunocytochemistry/ Immunofluorescence - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)This image is courtesy of an anonymous Abreview

    ab7671 staining (dog) MDCK II cells by ICC/IF.  Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C.  Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.

    See Abreview

  • Flow Cytometry - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Flow Cytometry - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)This image is courtesy of an anonymous Abreview

    ab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The  sample was incubated with the primary antibody 10 µg/ml in PBS  for 1 hour. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Immunocytochemistry/ Immunofluorescence - Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)This image is a courtesy of Anonymous Abreview

    ab7671 staining Sodium Potassium ATPase in 293 human embryonic kidneys cells. Cells were plated on an uncoated cover slip. One day after plating cells were washed twice with PBS and fixed with 4% paraformaldehyde with 4% sucrose at room temperature for 15 minutes. After washing once with PBS, 300µl wheat germ agglutinin (5µg/ml in PBS) conjugated to TexasRed were added and incubated at 4°C in darkness for 15 minutes. Cells were then washed twice with PBS and permeabilized with 0.5% saponin in PBS for 45 minutes at 4°C. Blocking was carried out with 10% fetal calf serum in PBS-saponin during first and second antibody incubation. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/500 dilution. After additional washing cover slips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    ICC/IF image of ab7671 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7671, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.
  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)
    All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1/1000 dilution

    Lane 1 : Parotid acinar cell lysate
    Lane 2 : Parotid acinar cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-Mouse polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 112 kDa


    Exposure time : 5 minutes

    See Abreview

References for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)

This product has been referenced in:
  • Watkins RJ  et al. A novel interaction between FRMD7 and CASK: evidence for a causal role in idiopathic infantile nystagmus. Hum Mol Genet 22:2105-18 (2013). WB ; Mouse . Read more (PubMed: 23406872) »
  • Roundhill EA & Burchill SA Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria. Br J Cancer 106:1224-33 (2012). Read more (PubMed: 22353810) »

See all 36 Publications for this product

Publishing research using ab7671 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 63 of 63 results for Abreviews and Q&A

100
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Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Caco^2 cell)
Specification Caco^2 cell
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Mar 28 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain sections (cortex))
Specification mouse brain sections (cortex)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Tri-Sodiumcitrate Dihydrate, pH6.0
Permeabilization Yes - Triton during staining
Blocking step PBS, 1%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
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Submitted Mar 04 2013

has crossreactivity with isoforms 2, 3, 4 been tested?


The lab let me know that we don't have more information about the immunogen for this antibody than what is on the datasheet. The only information we have is that the antibody reacts with alpha 1 subunit. There's no mention of it reacting with other i...

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Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Below Average
Abreviews
Application Western blot
Sample Human Cell lysate - other (HeLa lysate - post nuclear supernatant)
Loading amount 20 µg
Specification HeLa lysate - post nuclear supernatant
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jan 10 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Mixed glial culture)
Specification Mixed glial culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Submitted Dec 19 2012

the Ab we used is ab7671 (ms anti Na+/K+ ATPase).
We cultureed NIH3T3 cells in 4 chember slides and are transfected with 300 ng plasmid (we are study some functional gene). 48 hrs later we washed cell with 1X PBS 2 times, fixed with 4% PFA in PBS 15m...

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Thank you for your reply. The protocol you are using seems fine. My only recommendation would be that it may help to use 0.1% Tween 20 or Saponin for permeabilization instead of Triton X-100. Tween and Saponin will open pores in the plasma membrane, but w...

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1) Abcam product code ab 7671




2) Abcam order reference number or product batch number Lot GR 45417-2



3) Description of the problem: We tried Sodium Potassium ATPase antibody to label the plasma membrane ...

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Thank you for your enquiry regardingab7671 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise applie...

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Abcam IHC Questionnaire





It will take approximately 5 minutes to complete the questionnaire. Please fill-in all fields so that we can better assist you.



1) Abcam product code ab 7671

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Thank you for your enquiry regarding ab7671 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Could you please confirm the Abcam Order Number (or y...

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Lot number: GR45417-2
Inquiry: We tried Sodium Potassium ATPase antibody to label the plasma membrane in A549 cells. Unfortunately, the antibody did not stain the plasma membrane but staining was visible in intracellular vesicles. Cells were transfec...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used....

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For the ab65985 antibody, I used RIPA for membrane lysis. I blocked in both 5% milk and 5% BSA in separate trials. I had a problem in a strange background band up in the 220kDa range when using the 5% milk and had a very weak band of interest reading when...

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Thank you for contacting us.

I am sorry to hear of the troubles which you are having with this product. After review of the information which you have provided I have a few suggestions which may help.

1) It seems that the milk is r...

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Here are the details:
Po: 89144
Lot: GR45417
Invoice: 1341113

Thank you for your kind help.
Regards,

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ...

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The customer has made another attempt to change conditions but still didn't get good results.
We would very much appreciate it if you could arrange for ab2872as a replacementantibody.
Thank you very much for your kind help.
Regards,

Could you provide purchase order number for us to place an FOCR order?

Here is the blot image.
Thank you for the kind help.
Regards,

Thank you for sending the WB image.

It looks that the customer is having high background signal. Could you please confirm what the different lanes represent in the samples? It seems there are 4 sample lanes plus the MW marker lane.

I c...

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Dear Tech support
One of our customers has ordered ab7671.
The datasheet states not to boil the protein prior to gel running yet boiling the proteinis usually the standard protocol.

After running a boiled protein the customer has rece...

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Thank you for your enquiry.

We currently test in Western blot on cell and tissue lysates and heat our samples to 95-100C. The note was historical and made long time. It was very likely part of an Abreview feedback. In order to prevent further co...

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Thank you very much for the reply.
I'm working with membrane samples, would they also be heated to 95-100C for 10 minutes?
How come the datasheet for this product warns "Do not boil the protein samples, as boiling causes aggregation of the ...

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Thank you for your response.

Our Lab regularly test this product on whole cell lysates and heat the samples 95-100C for 10 minutes. The signal is very clear and distinctive. My personal experience is that plasmamebrane protein can be heating for...

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To whom it may concern,
I purchased the product "ab7671" alpha-1-Sodium-Potassium ATPase" and it states that samples shouldn't be boiled for westerns and
that they should be heated at 60 Celcius. How long can the samples be heated...

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Thank you for your enquiry and your interest in our products.

This note was made long time ago, it could be part of an Abreview feedback. We regularly test this antibody for Western blot in our Lab. I have discussed the enquiry with my colleague...

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Getting no signal staining human neuroblastoma cell lines H4 and N2A with ab7671; used 1:250 and 1:100 primary dilution overnight at 4 deg C; secondary is goat anti mouse and works with other mouse antibodies; use PFA for fixation; tried 3 different block...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and...

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Hi,

A customer has bought the AB7671, po 4506860537 and there was no ice when she received the shipment. She says that she probably can use it anyway, but she wants you to know that there was no ice at arrival.

Do you think that the ...

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Thank you for contacting us. Please forward this reassurance to your customer.

Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual...

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Please see below the response from the customer;
We are still not sure which is the antibody that would suit our needs but from the list that you have sent me , we are interested atab7671.
is it possible if you can ask Abcam if this antibody sta...

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Thank you for contacting us.

Yes, the antibody ab7671 will detect the protein in neurons.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our ...

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sorry, I should have been more specific... we are trying to find an antibody that stains Na K ATPase of nociceptors onhttp://en.wikipedia.org/wiki/A_delta_fibers or C fibres.. Hope that narrows it down a bit
I'm trying to find an antibody for our exp...

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Thank you for contacting us.

The antibody we have are ab7671; http://www.abcam.com/alpha-1-Sodium-Potassium-ATPase-antibody-464-6-Plasma-Membrane-Marker-ab7671.html;

The immunogen sequence of ab76509 is quite similar with rat sequence...

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Hi,   Thank you for your recommendation of this membrane specific antibody. We really need the same Western results as the figure shown in the following : to confirm the expression on membrane but not in cytosolic fraction in A549 cell line. Do you have...

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Thank you for contacting us.

This western blot image was submitted to us by a customer in one of our Abreviews, (http://www.abcam.com/index.html?datasheet=7671&tab=abreviews&intabreviewid=26638). While we do not know the exact protoco...

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Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - other (Huh7 membrane fraction)
Loading amount 30 µg
Specification Huh7 membrane fraction
Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 11 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary alveolar type II epithelial cells)
Specification Primary alveolar type II epithelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% saponin
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Apr 11 2012

Hi, Could you recommend a good antibody that I could use as loading control for western blot on cell membrane fractions? Thanks

Thank you for your enquiry.

Below is a selection of antibodies that are suitable for detecting the plasma membrane and nuclear envelope. They all work in western blot and I would review the datasheets to confirm which species they are tested ag...

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Dear Sir/Madam,

I want to bind antibody to either of the alpha or beta subunit of Na,K-ATPase in the cell membranes. My sample is purified native membrane from the outer medullar of rabbit kidney with the high density of Na,K-ATPase. I found on...

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Thank you for your email.

I am happy to confirm that ab7671 is specific for specific for Na,K-ATPase alpha 1 subunit and therefore should be suitable for your experiments.

We also have on anti beta Sodium Potassium ATPase antibody that...

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I am searching for an antibody that can be used to label live 3T3cells (unpermeabilized andunfixed).

Thank you for your phone call.

The only plasma membrane marker antibody that we currently sell in a buffer free from sodium azide is ab6529. This antibody will be suitable for use on mouse cells, but we have not validated it for unfixed or unper...

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1) No signal
2) 1:25 dilution, incubation time was overnight at 4'C in humidified chamber
3) Antigen retrieval step: (Citrate buffer, pH6, for 45'; blocked in 1% BSA + 0.1% Triton X in PBS for 30 minutes prior to primary antibody addition)
...

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Thank you for your reply and the additional information. This is very helpful to us for our internal
I have checked our stock, but we have, unfortunately, only a new lot for ab15081 - which I'd be happy to send you free of charge. For ab7671 and ab58...

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We recently received an order of 3 antibodies (ab58968, ab15081, ab7671) in the same shipment and have been trying to use them for IHC (rat sections, cadaveric, paraffin embedded).

Based upon the abreviews and online catalog information, we wer...

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Thank you for contacting us.
I am sorry to hear that all 3 antibodies are not performing as expected.
Before proceeding with the free of charge replacements, could you please let me know the following experimental details:
1) What was the e...

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DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Samples were membrane fraction from a total exctract of human cells. PRIMARY ANTIBODY Primary antibody diluted in TBS 1X (10 mM Tris pH 7.4 + 150 mM NaCl) at 1:5000 incubated or 1.5 h at RT or ove...

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I am sorry to hear that this antibody is not providing satisfactory results for your customer. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this...

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Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Western blot
Sample Rat Cell lysate - whole cell (Parotid acinar cells)
Loading amount 50 µg
Specification Parotid acinar cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
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Submitted Oct 31 2011

  Hello,  We intermittently have trouble detecting ATPase on Western blot (Antibody ab7671).   The lower molecular mass proteins (50-60 kDa) work well.  Ponceau protein staining indicated that there was much less high molecular weight protein on the membr...

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Thank you very much for contacting us. Indeed, it is happens that there are problems with the transfer of high molecular weight proteins. The exact reasons for this are unknown to me. I would however be happy to provide you with the following tips in orde...

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I would like to stain the plasma membrane of HEK293 cells. In the abcam list, I saw that you have 2 major options pan cadherin and Na K atpase antibodies but neither of them are tested for HEK cells? Do you see a nice staining for HEK cells? I...

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Thank you for contacting us. I recommend ab7671, Anti-alpha 1 Sodium Potassium ATPase antibody: Click here (or use the following: http://www.abcam.com/index.html?datasheet=7671). ...

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Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - other (adult human dermal fibroblasts)
Loading amount 10 µg
Specification adult human dermal fibroblasts
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 16 2011

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Average
Abreviews
Application Western blot
Sample Rat Cell lysate - other (Brain)
Loading amount 20 µg
Specification Brain
Treatment RIPA buffer
Gel Running Conditions Reduced Denaturing (12% Mini-Protean TGX)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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Submitted Dec 15 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Average
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mixed glia prepared from mouse brain)
Specification mixed glia prepared from mouse brain
Fixative Paraformaldehyde
Permeabilization Yes - 0,5%(w/v) saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Dec 13 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Average
Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (mixed glia prepared from mouse brain)
Loading amount 40 µg
Specification mixed glia prepared from mouse brain
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 01 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Non-small cell lung carcinoma)
Loading amount 10 µg
Specification Non-small cell lung carcinoma
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 23 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Western blot
Sample Rat Cell lysate - other (spinal cord cytoplasm, membrane and nuclear fracti)
Loading amount 12 µg
Specification spinal cord cytoplasm, membrane and nuclear fracti
Treatment Spinal cord 4hr post 300kdyne injury
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 08 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Western blot
Sample Mouse Tissue lysate - other (mouse salivary gland tissue)
Loading amount 20 µg
Specification mouse salivary gland tissue
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 19 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Western blot
Sample Human Cell lysate - other (MCF10A cell fractionation)
Loading amount 5 µg
Specification MCF10A cell fractionation
Gel Running Conditions Non-reduced Denaturing (7.5% SDS running gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 26 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Western blot
Sample Mouse Cell lysate - other (crude membrane extract from primary dermal fibrobl)
Loading amount 50 µg
Specification crude membrane extract from primary dermal fibrobl
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Feb 19 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Average
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293 human embryonic kidney)
Specification 293 human embryonic kidney
Fixative 4%(w/v) Paraformaldehyde + 4%(w/v) sucrose
Permeabilization Yes - 0,5%(w/v) saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Feb 19 2010

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Inconclusive
Abreviews
Application Western blot
Sample Zebrafish Tissue lysate - other (Gill, whole fish)
Loading amount 50 µg
Specification Gill, whole fish
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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Submitted Feb 12 2010

Flow Cytometry abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Flow Cytometry
Sample Human Cell (Fibrosarcoma HT1080)
Specification Fibrosarcoma HT1080
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy no gating
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Submitted Jul 22 2009

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK II)
Specification MDCK II
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Nov 12 2007

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Poor
Abreviews
Application Western blot
Sample Xenopus laevis Cell lysate - other (oocytes)
Loading amount 1 cells
Specification oocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Aug 03 2007

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - other (SD1 and REH cell plasma membrane prep)
Loading amount 10 µg
Specification SD1 and REH cell plasma membrane prep
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
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Submitted Mar 27 2007

I would like to use a loading control for crude membrane preparation from (human) whole adipose tissue. Do you have any suggestions? I thought that the (Na+K)ATPase antibody may be an option but got no bands in 7-50 ug (total protein) applications. Do you...

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Thank you for your enquiry. We have received previous enquiries of this nature. I would like to recommend that you use a nuclear loading control. For example an antibody that targets RNA polII. Alternatively a structural nuclear protein such as Topois...

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Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Western blot
Sample Human Cell lysate - other (lung and foreskin fibroblast cells)
Specification lung and foreskin fibroblast cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Feb 15 2006

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

 Good
Abreviews
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Oocytes)
Specification Oocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Submitted Jan 26 2006

I am interesting in buying an antibody that is specific to Sodium potassium ATPase, that is specific to a peptide of this protein that lies within the cytoplasm. I believe that most commercial ATPase antibodies are specific to regions that are exposed on...

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Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from ra...

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Can you specifiy what "Do not boil samples" means. Does it mean I cannot heat the sample to 100?C but instead to 60?C or 80?C or what is the limit? And did you verify if it reacts with mitochondrial proteins like described on the homepage by another user?...

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Thank you for your enquiry regarding ab7671. The temperature of 60 degrees Celcius appears to work fine so I would recommend using this temperature. We have not further tested the antibody for reactivity to other mitochondrial proteins, as soon as we ...

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Customer is using this antibody in WB with human neutrophil samples and is doing a membrane fractionation. He is seeing 2 bands at approx. 50-75 kDa but nothing at 100 kDa. Has this been reported before? Is there perhaps a problem with the membrane fracti...

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Thank you for your enquiry and patience. I checked with our source for this antibody and there have not been reports of bands at 50-75 kDa being detected. I think that there may possibly be a problem with your membrane fractionation. I would suggest...

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I contacted you last week, because I had some problems with the antibody ab7671 (anti-Na,K-ATPase). Your supplier answered that I should try and leave out boiling my samples prior to loading it on the gel, as the Na,K-ATPase might aggregate. So I tried it...

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Thank you for your e-mail, I am very glad you are having successful results with the antibody. Would you like to submit this feedback with this lovely image, we give Abcam loyalty points as rewards for reviews (you can use them on Amazon gift vouchers)...

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BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 61772 DESCRIPTION OF THE PROBLEM Sometimes I see two bands above the 175 kDa protein marker, and in other preparations I see no bands at all. Please see Additional Notes. SAMPLE Whole ti...

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We have just received the following information from the supplier: "I believe the problem is with the sample preparation. We recommend that you do not boil the samples as this causes aggregation of the protein resulting in a band at ~150 kDa (but I assu...

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BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 61772 DESCRIPTION OF THE PROBLEM Sometimes I see two bands above the 175 kDa protein marker, and in other preparations I see no bands at all. Please see Additional Notes. SAMPLE Whole ti...

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I'm sorry to hear you are having a problem with ab7671. Thank you very much for taking the time to fill in our questionnaire, it is very useful. Could you please clarify which samples (and what subcellular fractions) gave you the bands and which did n...

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We just ordered two primary antibodies from Abcam, ab7671 and ab7639. I was wondering whether abcam has the original proteins, Sodium-Potassium ATPase and Lactate Dehydrogenase, that inteact with those two antibodies. I appreciate if you could assist us...

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Thank you for your email. I'm afraid that we don't sell Sodium-Potassium ATPase or Lactate Dehydrogenase. Just to let you know, you can read some feedback posted by customers who have used these antibodies in Western blotting by clicking on the reviews ...

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I just received our order of antibodies from Abcam today. Item #ab7671 should have 50ul, but I can only get 20ul out from the tube. I'm wondering about that. Look forward to your reply.

Thank you for your email and I apologize for the shortage that you received in your vial of ab7671. I'm sending you a replacement vial free of charge. For your record it is being sent to your attention on order number 57915 and you should receive it Wed...

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I would like to use ATPase (Na+K)antibody (ab7671) to mark the plasma membrane in COS-1 cell lysate. Do you have any references about using ab7671 for this purpose?

Unfortunately, we are not aware of any publications that feature the use of this antibody.

I want to use ATPase (Na+K)antibody (ab7671) to mark the plasma membrane in living cells .More specifically i do not want to lyse the cells. Further as mentioned in the data sheet it can be used as a marker for plasma membrane. Finally my query is , can...

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Thank you for your enquiry and your interest in our products. This antibody has been tested and characterised for Western blot application and as you probably for this assay cell lysate needs to be applied. We are very sorry to inform you that ...

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The first time I used the antibody for WB it worked fine.I had a band in my whole cell extract, a less intense band in my nuclei extract, probably due to contamination and no other visible bands in cytoplasmic and mitochondrial extract. Now it reacts very...

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Thank you for your enquiry. I am very sorry to hear that you are having difficulty with this antibody. I have read through your message but it is not clear to me what you mean by first time and second time. Do you use different cell lysate preparations...

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What's the recognized epitope? Does it recognize Na/K ATPase alpha2 and alpha3 isoforms? Thank you.

NB 300-146 is only specific for human Na, K-ATPase alpha-1 subunit.

Does this antibody react with human ATPase (it is unclear from the spec sheet, since the latter indicates rabbit ATPase as the antigen used to generate the antibody).

This particular antibody recognizes porcine and rat. Other species have not, yet, been tested.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"