Anti-alpha 1 Spectrin antibody [AF10] (ab86184)
Key features and details
- Mouse monoclonal [AF10] to alpha 1 Spectrin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-alpha 1 Spectrin antibody [AF10]
See all alpha 1 Spectrin primary antibodies -
Description
Mouse monoclonal [AF10] to alpha 1 Spectrin -
Host species
Mouse -
Specificity
ab86184 is specific to the erythroid alpha 1 Spectrin. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human alpha 1 Spectrin. Ghost proteins of human red blood cells.
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General notes
ab86184 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab86184 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
Clonality
Monoclonal -
Clone number
AF10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 280 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Predicted molecular weight: 280 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. -
Involvement in disease
Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive. -
Sequence similarities
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 21 spectrin repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. - Information by UniProt
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Database links
- Entrez Gene: 6708 Human
- Omim: 182860 Human
- SwissProt: P02549 Human
- Unigene: 119825 Human
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Alternative names
- Alpha I spectrin antibody
- EL 2 antibody
- EL2 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab86184 has not yet been referenced specifically in any publications.