Recombinant Anti-alpha 1 Spectrin antibody [EPR9300] (ab139403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9300] to alpha 1 Spectrin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-alpha 1 Spectrin antibody [EPR9300]
See all alpha 1 Spectrin primary antibodies -
Description
Rabbit monoclonal [EPR9300] to alpha 1 Spectrin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human alpha 1 Spectrin aa 2300-2400. The exact sequence is proprietary.
-
Positive control
- K562, Human fetal heart and fetal liver lysates; K562 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9300 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 280 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 280 kDa. |
Target
-
Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. -
Involvement in disease
Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive. -
Sequence similarities
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 21 spectrin repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. - Information by UniProt
-
Database links
- Entrez Gene: 6708 Human
- Entrez Gene: 20739 Mouse
- Entrez Gene: 289257 Rat
- Omim: 182860 Human
- SwissProt: P02549 Human
- SwissProt: P08032 Mouse
- Unigene: 119825 Human
- Unigene: 200611 Mouse
-
Alternative names
- Alpha I spectrin antibody
- EL 2 antibody
- EL2 antibody
see all
Images
-
All lanes : Anti-alpha 1 Spectrin antibody [EPR9300] (ab139403) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 280 kDa -
Intracellular flow cytometric analysis of permeabilized K562 cells labelling alpha 1 Spectrin with ab139403 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab139403 has been referenced in 3 publications.
- Uaesoontrachoon K et al. Orthogonal analysis of dystrophin protein and mRNA as a surrogate outcome for drug development. Biomark Med 13:1209-1225 (2019). PubMed: 31379197
- Kooijmans SAA et al. Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach. Nanoscale 10:2413-2426 (2018). PubMed: 29334397
- Vorselen D et al. The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis. Nat Commun 9:4960 (2018). PubMed: 30470753