Anti-Alpha 1 Acid Glycoprotein antibody (ab52691)

Overview

  • Product nameAnti-Alpha 1 Acid Glycoprotein antibody
    See all Alpha 1 Acid Glycoprotein primary antibodies
  • Description
    Mouse polyclonal to Alpha 1 Acid Glycoprotein
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: ENGTISRYVG GQEHFAHLLI LRDTKTYMLA FDVNDEKNWG LSVYADKPET TKEQLGEFYE ALDCLRIPKS DVVYTDWKKD KCEPLEKQHE KERKQEEGES , corresponding to amino acids 102-202 of Human Alpha 1 Acid Glycoprotein

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 24 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionAppears to function in modulating the activity of the immune system during the acute-phase reaction.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1AG1_HUMAN antibody
    • AGP 1 antibody
    • AGP A antibody
    • AGP antibody
    • AGP1 antibody
    • Alpha 1 acid glycoprotein antibody
    • Alpha-1-acid glycoprotein 1 antibody
    • Epididymis secretory sperm binding protein Li 153w antibody
    • HEL S 153w antibody
    • OMD 1 antibody
    • ORM antibody
    • ORM1 antibody
    • Orosomucoid 1 antibody
    • Orosomucoid-1 antibody
    see all

Anti-Alpha 1 Acid Glycoprotein antibody images

  • All lanes : Anti-Alpha 1 Acid Glycoprotein antibody (ab52691) at 1/1000 dilution

    Lane 1 : Left: The negative control lane of ~20ug of a total protein extract from E coli with ~50ng to 100 ng of a fusion protein of an irrelevant antigen.
    Lane 2 : Right: test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (antigen fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 24 kDa

References for Anti-Alpha 1 Acid Glycoprotein antibody (ab52691)

ab52691 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"