ICC/IF image of ab58291 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58291 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alpha 1 Acid Glycoprotein antibody (ab58291) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach carcinoma tissue.
Western blot - Alpha 1 Acid Glycoprotein antibody (ab58291)
Predicted band size : 24 kDa Alpha 1 Acid Glycoprotein antibody (ab58291) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
References for Anti-Alpha 1 Acid Glycoprotein antibody (ab58291)
This product has been referenced in:
Clements RT et al. Pilot proteomic profile of differentially regulated proteins in right atrial appendage before and after cardiac surgery using cardioplegia and cardiopulmonary bypass. Circulation118:S24-31 (2008).
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