Overview

  • Product nameAnti-Alpha 1 Acid Glycoprotein antibody
    See all Alpha 1 Acid Glycoprotein primary antibodies
  • Description
    Mouse monoclonal to Alpha 1 Acid Glycoprotein
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 18-202 of Human Alpha 1 Acid Glycoprotein

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab58291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 1 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAppears to function in modulating the activity of the immune system during the acute-phase reaction.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A1AG1_HUMAN antibody
      • AGP 1 antibody
      • AGP A antibody
      • AGP antibody
      • AGP1 antibody
      • Alpha 1 acid glycoprotein antibody
      • Alpha-1-acid glycoprotein 1 antibody
      • Epididymis secretory sperm binding protein Li 153w antibody
      • HEL S 153w antibody
      • OMD 1 antibody
      • ORM antibody
      • ORM1 antibody
      • Orosomucoid 1 antibody
      • Orosomucoid-1 antibody
      see all

    Anti-Alpha 1 Acid Glycoprotein antibody images

    • ICC/IF image of ab58291 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58291 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Alpha 1 Acid Glycoprotein antibody (ab58291) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach carcinoma tissue.


    • Predicted band size : 24 kDa
      Alpha 1 Acid Glycoprotein antibody (ab58291) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.

    References for Anti-Alpha 1 Acid Glycoprotein antibody (ab58291)

    This product has been referenced in:
    • Clements RT  et al. Pilot proteomic profile of differentially regulated proteins in right atrial appendage before and after cardiac surgery using cardioplegia and cardiopulmonary bypass. Circulation 118:S24-31 (2008). WB ; Human . Read more (PubMed: 18824761) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"