Anti-alpha 1d Adrenergic Receptor antibody (ab166925)
Key features and details
- Goat polyclonal to alpha 1d Adrenergic Receptor
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-alpha 1d Adrenergic Receptor antibody
See all alpha 1d Adrenergic Receptor primary antibodies -
Description
Goat polyclonal to alpha 1d Adrenergic Receptor -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Cynomolgus monkey, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human alpha 1 Adrenergic Receptor/ADRA1 aa 266-278 (Cysteine residue).
Sequence:SSTKAKGHNPRSS
Database link: NP_000670.1 -
Positive control
- WB: Human, mouse, pig and rat heart lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab166925 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 57 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 57 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1D sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 539543 Cow
- Entrez Gene: 552897 Dog
- Entrez Gene: 146 Human
- Entrez Gene: 11550 Mouse
- Entrez Gene: 552898 Pig
- Entrez Gene: 100009398 Rabbit
- Entrez Gene: 29413 Rat
- Omim: 104219 Human
see all -
Alternative names
- ADA1D_HUMAN antibody
- Adra1 antibody
- Adra1a antibody
see all
Images
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All lanes : Anti-alpha 1d Adrenergic Receptor antibody (ab166925) at 0.1 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Mouse heart lysate (in RIPA buffer)
Lane 3 : Rat heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDaPrimary incubation was 1 hour.
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Anti-alpha 1d Adrenergic Receptor antibody (ab166925) at 0.1 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 57 kDaPrimary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (1)
ab166925 has been referenced in 1 publication.
- Tang ZR et al. Terazosin reduces steroidogenic factor 1 and upregulates heat shock protein 90 expression in LH-induced bovine ovarian theca cells. Free Radic Biol Med 163:190-195 (2021). PubMed: 33352221