This antibody gave a positive signal in the following Tissue lysates: Human Spinal cord; Human Colon; Hu Brain Frontal lobe (Alzheimers); Hu Plasma total protein.
This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
Sequence similaritiesBelongs to the serpin family.
DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
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Cell growth-inhibiting gene 24/25 protein antibody
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Growth inhibiting protein 25 antibody
Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
Serine proteinase inhibitor clade A member 3 antibody
Serpin A3 antibody
Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
Anti-alpha 1 Antichymotrypsin antibody images
Western blot - Anti-alpha 1 Antichymotrypsin antibody (ab107362)
All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab107362) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188) Lane 2 : Human colon tissue lysate - total protein (ab30051) Lane 3 : Brain Frontal Lobe (Human)Tissue Lysate - Alzheimer Lane 4 : Human Plasma Total Protein Lysate
IHC image of alpha 1 Antichymotrypsin staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107362, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-alpha 1 Antichymotrypsin antibody (ab107362)
has not yet been referenced specifically in any publications.
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