Anti-alpha 1 Antichymotrypsin antibody (ab88527)

Overview

  • Product nameAnti-alpha 1 Antichymotrypsin antibody
    See all alpha 1 Antichymotrypsin primary antibodies
  • Description
    Mouse polyclonal to alpha 1 Antichymotrypsin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human alpha 1 Antichymotrypsin protein (NP_001076.2).

  • Positive control
    • WB: HeLa cell lysate and alpha 1 Antichymotrypsin transfected 293T cell lysate IF: HeLa cell

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • SERPINA3 antibody
    • AACT antibody
    • AACT_HUMAN antibody
    • ACT antibody
    • Alpha-1-antichymotrypsin His-Pro-less antibody
    • Antichymotrypsin antibody
    • Cell growth inhibiting gene 24/25 protein antibody
    • Cell growth-inhibiting gene 24/25 protein antibody
    • GIG24 antibody
    • GIG25 antibody
    • Growth inhibiting protein 24 antibody
    • Growth inhibiting protein 25 antibody
    • MGC88254 antibody
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
    • Serine proteinase inhibitor clade A member 3 antibody
    • Serpin A3 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
    • SERPINA3 antibody
    see all

Anti-alpha 1 Antichymotrypsin antibody images

  • Anti-alpha 1 Antichymotrypsin antibody (ab88527) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab88527) at 1 µg/ml

    Lane 1 : alpha 1 Antichymotrypsin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab88527 at 10 µg/ml staining alpha 1 Antichymotrypsin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.

References for Anti-alpha 1 Antichymotrypsin antibody (ab88527)

ab88527 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88527.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"