Anti-alpha 1 Antichymotrypsin antibody (ab9374)


  • Product nameAnti-alpha 1 Antichymotrypsin antibody
    See all alpha 1 Antichymotrypsin primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Antichymotrypsin
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all MammalsDoes not react with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to alpha 1 Antichymotrypsin. Alpha-1-antichymotrypsin isolated from human serum.



Our Abpromise guarantee covers the use of ab9374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/25 - 1/50. ABC method.
IHC-P 1/25 - 1/50. ABC method.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693).

For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) as Capture.

WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa).

Assay Dependent


  • FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • SERPINA3 antibody
    • AACT antibody
    • AACT_HUMAN antibody
    • ACT antibody
    • Alpha-1-antichymotrypsin His-Pro-less antibody
    • Antichymotrypsin antibody
    • Cell growth inhibiting gene 24/25 protein antibody
    • Cell growth-inhibiting gene 24/25 protein antibody
    • GIG24 antibody
    • GIG25 antibody
    • Growth inhibiting protein 24 antibody
    • Growth inhibiting protein 25 antibody
    • MGC88254 antibody
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
    • Serine proteinase inhibitor clade A member 3 antibody
    • Serpin A3 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
    • SERPINA3 antibody
    see all

Anti-alpha 1 Antichymotrypsin antibody images

  • Standard Curve for alpha 1 Antichymotrypsin (Analyte: alpha 1 Antichymotrypsin protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) at 0.5µg/ml.
  • All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab9374) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 48 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel: with primary antibody at 2 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-alpha 1 Antichymotrypsin antibody (ab9374) at 1 µg/ml + Natural Human alpha 1 Antichymotrypsin protein (ab80517) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds


References for Anti-alpha 1 Antichymotrypsin antibody (ab9374)

ab9374 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The antibody is suspended in PDB and it contains BSA. PDB buffer is PBS supplemented with 1.5 mM MgCl2, 0.1 mM dithiothreitol, 0.01% bovine serum albumin. I have updated this on the datasheet for future reference. Pl...

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Thank you for your email. This antibody has not yet been tested in ELISA. So far, it has only been characterized in Immunohistochemistry - both formalin-fixed paraffin-embedded and frozen sections. If you have any more questions, please contact us again.

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