Recombinant
RabMAb

Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

Overview

  • Product name
    Anti-alpha 1 Antitrypsin antibody [EPR9090]
    See all alpha 1 Antitrypsin primary antibodies
  • Description
    Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region of Human alpha 1 Antitrypsin (UniProt ID: P01009).

  • Positive control
    • WB: Human serum and fetal liver lysates. ICC/IF: HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa).
ICC/IF 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
      Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
    • Tissue specificity
      Ubiquitous. Expressed in leukocytes and plasma.
    • Involvement in disease
      Alpha-1-antitrypsin deficiency
    • Sequence similarities
      Belongs to the serpin family.
    • Domain
      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Post-translational
      modifications
      N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
      Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
    • Cellular localization
      Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all

    Images

    • ab166610 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166610 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 46 kDa
      Observed band size: 52 kDa (why is the actual band size different from the predicted?)

    References

    ab166610 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Serum (serum (loading in microliter per lane))
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    2 µg
    Specification
    serum (loading in microliter per lane)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Georg Haase

    Verified customer

    Submitted Feb 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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