FITC Anti-alpha 1 Antitrypsin antibody (ab19170)
Key features and details
- FITC Goat polyclonal to alpha 1 Antitrypsin
- Suitable for: ICC, ELISA, ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-alpha 1 Antitrypsin antibody
See all alpha 1 Antitrypsin primary antibodies -
Description
FITC Goat polyclonal to alpha 1 Antitrypsin -
Host species
Goat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with alpha 1 antitrypsin. This antibody may cross react with alpha 1 antitrypsin light chain from other species. -
Tested applications
Suitable for: ICC, ELISA, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab19170 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 21060150
ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 21060150 ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
Tissue specificity
Ubiquitous. Expressed in leukocytes and plasma. -
Involvement in disease
Alpha-1-antitrypsin deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Post-translational
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
Cellular localization
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
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Alternative names
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab19170 has been referenced in 4 publications.
- O'Brien ME et al. Activation of complement component 3 is associated with airways disease and pulmonary emphysema in alpha-1 antitrypsin deficiency. Thorax 75:321-330 (2020). PubMed: 31959730
- Bergin DA et al. The circulating proteinase inhibitor a-1 antitrypsin regulates neutrophil degranulation and autoimmunity. Sci Transl Med 6:217ra1 (2014). PubMed: 24382893
- Carroll TP et al. Evidence for unfolded protein response activation in monocytes from individuals with alpha-1 antitrypsin deficiency. J Immunol 184:4538-46 (2010). ICC/IF ; Human . PubMed: 20228200
- Bergin DA et al. a-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8. J Clin Invest 120:4236-50 (2010). ICC/IF, Flow Cyt ; Human . PubMed: 21060150