• Product nameAnti-alpha 1 Antitrypsin antibody [G11]
    See all alpha 1 Antitrypsin primary antibodies
  • Description
    Mouse monoclonal [G11] to alpha 1 Antitrypsin
  • SpecificityRecognises alpha 1-antitrypsin. Does not react with other human plasma proteins.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein.



Our Abpromise guarantee covers the use of ab9400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.


  • FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificityUbiquitous. Expressed in leukocytes and plasma.
  • Involvement in diseaseAlpha-1-antitrypsin deficiency
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localizationSecreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Dom1 antibody
    • PI antibody
    • PI1 antibody
    • PRO2275 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • Serpina1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Anti-alpha 1 Antitrypsin antibody [G11] images

  • Western blot using ab9400 at a concentration of 0.5 µg/ml.
    Lane 1 - purified alpha 1 antitrypsin
    lane 2 - human plasma, 2 µl
    lane S - standard proteins
    A - SDS-PAGE with Coomasie staining
    B - immunoblot with ab9400.

  • Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution + Albumin depleted human patient sera, tissue lysate at 50 µg/ml

    HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution

    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Mr John Andersen

    See Abreview

References for Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)

This product has been referenced in:
  • Burgener A  et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . Read more (PubMed: 23449785) »
  • Lu J  et al. A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro. Mol Ther 21:954-63 (2013). WB ; Human . Read more (PubMed: 23459514) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human patient sera)
Loading amount 50 µg
Specification Human patient sera
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. John Andersen

Verified customer

Submitted Jul 25 2008