This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR3176514-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000.
1/1000 - 1/5000.
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Ubiquitous. Expressed in leukocytes and plasma.
Involvement in disease
Belongs to the serpin family.
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
Serine protease inhibitor 1-1 antibody
Serine protease inhibitor A1a antibody
Serpin A1 antibody
Serpin A1a antibody
Serpin peptidase inhibitor clade A member 1 antibody
Short peptide from AAT antibody
Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)
Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin lane 2 - human plasma, 2 µl lane S - standard proteins
A - SDS-PAGE with Coomasie staining B - immunoblot with ab9400.
Western blot using ab9400 at a concentration of 0.5 µg/ml. Lane 1 - purified alpha 1 antitrypsin lane 2 - human plasma, 2 µl lane S - standard proteins A - SDS-PAGE with Coomasie staining B - immunoblot with ab9400.
Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)This image is courtesy of an Abreview submitted by Mr John Andersen
Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution + Albumin depleted human patient sera, tissue lysate at 50 µg/ml
Secondary HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution