Anti-alpha 1 Antitrypsin antibody (HRP) (ab8768)

Overview

  • Product name
    Anti-alpha 1 Antitrypsin antibody (HRP)
    See all alpha 1 Antitrypsin primary antibodies
  • Description
    Sheep polyclonal to alpha 1 Antitrypsin (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human α1-antitrypsin purified from human plasma and shown to be pure by IEP.

  • General notes

    Horseradish peroxidase by the periodate method

Properties

Applications

Our Abpromise guarantee covers the use of ab8768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ELISA
  • Application notes
    ELISA: To give an A450nm = 1.0 using wells coated with human a1-antitrypsin at 5µg/mL andtetramethylbenzidine as the chromogen: 1/6400 – 1/12800
    The titres reported should be used as guidelines for determining the working dilution in the users system.
    The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent non-specific binding in ELISA.
    It may also be advantageous in other assay systems.
  • Target

    • Function
      Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
      Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
    • Tissue specificity
      Ubiquitous. Expressed in leukocytes and plasma.
    • Involvement in disease
      Alpha-1-antitrypsin deficiency
    • Sequence similarities
      Belongs to the serpin family.
    • Domain
      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Post-translational
      modifications
      N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
      Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
    • Cellular localization
      Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all

    References

    ab8768 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Purified protein
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0,1%
    Type
    Direct
    Username

    Dr.

    Verified customer

    Submitted Dec 07 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up