Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)

Overview

  • Product name
    Anti-alpha 1 Catenin antibody [EP1793Y]
    See all alpha 1 Catenin primary antibodies
  • Description
    Rabbit monoclonal [EP1793Y] to alpha 1 Catenin
  • Tested applications
    Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human alpha 1 Catenin.

  • Positive control
    • HeLa cell lysate, human breast carcinoma tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab51032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IP 1/50.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificity
    Expressed ubiquitously in normal tissues.
  • Sequence similarities
    Belongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • MDPT2 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Anti-alpha 1 Catenin antibody [EP1793Y] images

  • Ab51032 (1:100) staining human alpha 1 Catenin in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
  • ICC/IF image of ab51032 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51032, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (IgG H+L; ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • ICC/IF image of Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) stained mouse ES cells. The cells were fixed in 1:1 methanol/acetone, permeabilized using 0.1% Triton X, and blocked with 1% serum in PBS for 30 minutes. The cells were then incubated with ab51032 at a 1/100 dilution for 16 hours at 4oC. The secondary antibody was a Goat Anti-Rabbit Alexa Fluor 488 (IgG H&L;  ab150077) used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing MCF7 cells stained with ab51032 (red line). The cells were fixed with 4% PFA (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51032, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-rabbit DyLight® 488 (IgG H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa


    Exposure time : 1 minute

References for Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)

This product has been referenced in:
  • Qiao B  et al. MicroRNA-27a-3p Modulates the Wnt/ß-Catenin Signaling Pathway to Promote Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Stem Cells by Targeting SFRP1. Sci Rep 7:44688 (2017). WB ; Human . Read more (PubMed: 28425477) »
  • Zeng LS  et al. Overexpressed HDAC4 is associated with poor survival and promotes tumor progression in esophageal carcinoma. Aging (Albany NY) 8:1236-49 (2016). WB . Read more (PubMed: 27295551) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
Undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
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Submitted Feb 01 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Undifferentiated Human ES cells on MEFs)
Gel Running Conditions
Non-reduced Denaturing (12.5% SDS gel)
Loading amount
20 µg
Specification
Undifferentiated Human ES cells on MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Feb 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Submitted Nov 18 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Nov 13 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (hESC, MCF7, MET5A)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
hESC, MCF7, MET5A
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Aug 18 2015

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.5% v/v tween20 in PBS
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.5% v/v tween20 in PBS
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Submitted Apr 30 2014

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Dec 30 2013

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol/acetone 1:1
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Submitted Dec 20 2013

Thank you for contacting us.

This product was tested with rat, human and mouse samples only; xenopus was never tried. We can however predict, it will be fine for Xenopus as well because the N-terminal end of Xenopus protein (http://www.uniprot...

Read More

Thank you for contacting Abcam. I would recommend ab7671, the alpha 1 Sodium Potassium ATPase antibody as a marker for your membrane fraction. Please let me know if there is anything else I can help you with.

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