Anti-alpha 1 Catenin antibody - N-terminal (ab189310)

Overview

  • Product name
    Anti-alpha 1 Catenin antibody - N-terminal
    See all alpha 1 Catenin primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Catenin - N-terminal
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human alpha 1 Catenin (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35221

  • Positive control
    • Human kidney and mouse brain tissue; MCF-7 cells; mouse bladder lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab189310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 100 kDa.

Target

  • Function
    Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificity
    Expressed ubiquitously in normal tissues.
  • Sequence similarities
    Belongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • MDPT2 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Kidney Tubules labeling alpha 1 Catenin with ab189310 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain labeling alpha 1 Catenin with ab189310 at 5 µg/ml followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of MCF-7 cells (bottom histogram) labeling alpha 1 Catenin with ab189310 at 1/10 dilution and a negative control (top). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-alpha 1 Catenin antibody - N-terminal (ab189310) at 1/1000 dilution + mouse bladder tissue lysate at 35 µg

    Predicted band size : 100 kDa

References

ab189310 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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