Anti-alpha 1 Fetoprotein antibody [2A9] (ab114028)

Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody [2A9]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Mouse monoclonal [2A9] to alpha 1 Fetoprotein
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human alpha 1 Fetoprotein produced in HEK293T cells (NP_001125).

  • Positive control
    • Human liver tissue; HEK293T cell lysate transfected with pCMV6-ENTRY alpha 1 Fetoprotein cDNA; HepG2, HeLa, MCF7, MDCK, Jurkat, COS7 and A549 cell extracts; HEK293T cells transfected with pCMV6-ENTRY alpha 1 Fetoprotein overexpress plasmid; Jurkat cells; COS7 cells transiently transfected by pCMV6-ENTRY alpha 1 Fetoprotein.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab114028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 69 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody [2A9] images

  • All lanes : Anti-alpha 1 Fetoprotein antibody [2A9] (ab114028) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY alpha 1 Fetoprotein cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 69 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206622)
  • All lanes : Anti-alpha 1 Fetoprotein antibody [2A9] (ab114028) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 69 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206622)
  • ab114028 at 1/150 dilution staining alpha 1 Fetoprotein in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab114028 at 1/100 dilution staining alpha 1 Fetoprotein in HEK293T cells transfected with either pCMV6-ENTRY alpha 1 Fetoprotein overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab114028 at 1/100 dilution staining alpha 1 Fetoprotein in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab114028 at 1/100 dilution staining alpha 1 Fetoprotein in COS7 cells transiently transfected with pCMV6-ENTRY alpha 1 Fetoprotein by Immunofluorescence.

References for Anti-alpha 1 Fetoprotein antibody [2A9] (ab114028)

ab114028 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab114028.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"