Anti-alpha 1 Fetoprotein antibody [4A3] (ab10071)


  • Product nameAnti-alpha 1 Fetoprotein antibody [4A3]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Mouse monoclonal [4A3] to alpha 1 Fetoprotein
  • Specificityab10071 and 10072 react with different determinants of human a-fetoprotein. They do not react with albumin.
  • Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (purified) (Human).

  • General notesConcentration varies from lot to lot and can be provided on request.


Associated products


Our Abpromise guarantee covers the use of ab10071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5H7] to alpha 1 Fetoprotein (HRP) (ab10072). Can be used as capture antibody.
ELISA Use at an assay dependent dilution.


  • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

References for Anti-alpha 1 Fetoprotein antibody [4A3] (ab10071)

This product has been referenced in:
  • Schmelzer E  et al. Three-dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells. Tissue Eng Part A 16:2007-16 (2010). Sandwich ELISA ; Human . Read more (PubMed: 20088704) »

See 1 Publication for this product

Product Wall

Thanks for your reply, and I am sorry that I don't have a better explanation for this problem. If there is anything else that we can do for you, please let me know and I'll be happy to help.

I just wanted to thank you for completing our follow-up survey recently, and I'm sorry to see that this problem persisted even with the replacement vial of antibody. Since the results are still not satisfactory, I would be happy to issue a credit or re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (Liver cell culture)
Specification Liver cell culture
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type Sandwich (Capture)

Dr. Eva Schmelzer

Verified customer

Submitted Jul 20 2010

Thank you for your enquiry. Unless a dilution is listed on the data sheet, we do not know the dilution to use for sandwich ELISA. A few things to keep in mind are that typically you do not need a lot of antibody to do an ELISA, just enough to coat the ...

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