Anti-alpha 1 Fetoprotein antibody (ab115133)

Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Fetoprotein
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse alpha 1 Fetoprotein Receptor.

  • Positive control
    • This antibody gave a positive signal in Human Fetal Liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 67 kDa).

Target

  • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody images

  • Anti-alpha 1 Fetoprotein antibody (ab115133) at 1 µg/ml + Liver (Human) Tissue Lysate - fetal normal tissue (ab29890) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 69 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

References for Anti-alpha 1 Fetoprotein antibody (ab115133)

ab115133 has not yet been referenced specifically in any publications.

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