Anti-alpha 1 Fetoprotein antibody (ab46799)

Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Fetoprotein
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in C-terminus of human AFP.

  • Positive control
    • HepG2 cell lysate; Human fetal liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab46799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
    • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
    • Post-translational
      modifications
      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Afp antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    Anti-alpha 1 Fetoprotein antibody images

    • ab46799 staining alpha 1 Fetoprotein in cat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/50 in PBS + 1X casein) for 1 hour at 25°C. An undiluted HRP-conjugated horse anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • ab46799 staining alpha 1 Fetoprotein in Mouse embryonic liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in 1% BSA in TBS) for 1 hour at 23°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.

      See Abreview

    • Anti-alpha 1 Fetoprotein antibody (ab46799) at 1/50000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled, at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References for Anti-alpha 1 Fetoprotein antibody (ab46799)

    This product has been referenced in:
    • Qi X  et al. Development of inCVAX, In situ Cancer Vaccine, and Its Immune Response in Mice with Hepatocellular Cancer. J Clin Cell Immunol 7:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 27656328) »
    • Ambade A  et al. Alcoholic hepatitis accelerates early hepatobiliary cancer by increasing stemness and miR-122-mediated HIF-1a activation. Sci Rep 6:21340 (2016). IHC-P . Read more (PubMed: 26888602) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Liver tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC in a PT link
    Permeabilization No
    Specification Liver tumor
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 24 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Sample Cat Tissue sections (liver)
    Specification liver
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 28 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Embryonic Liver)
    Specification Embryonic Liver
    Fixative Formalin
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 04 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (embryoid body)
    Specification embryoid body
    Fixative Formaldehyde
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Username

    Ms. Han-Ni Cheng

    Verified customer

    Submitted Sep 19 2012


    I am a bit confused regarding this inquiry: The customer has recieved a a discount code for one antibody to test. She submitted the Abreview, therefore, she can use thiscode to buyone product for free.

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    I found the review of your customer, and it looks like that my colleague is working on it. she also left a note that the discount code is prolonged. Please let us know if your customer should come any problems in the future with the new code.

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    DISCOUNT CODE: XXXXX
    Expiration date: XXXXX

    I am very pleased to hear your customerwould like to test ab46799 Anti-alpha 1 Fetoprotein antibody in ICC. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem ...

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    Thank you for your patience in this matter. The originator of ab46799 (alpha 1 Fetoprotein antibody) has informed us that this product was tested in WB (general protocol) with rat brain lysate. A WB image is available on our datasheet (human sample...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"