Anti-alpha 1 Fetoprotein antibody (ab54745)

Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Mouse monoclonal to alpha 1 Fetoprotein
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 500-610 of Human alpha 1 Fetoprotein

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab54745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P Use a concentration of 3 µg/ml.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to alpha 1 Fetoprotein (ab8201). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody images

  • alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 69 kDa
    alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ab54745 at 10 ug/ml staining alpha Fetoprotein in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml

Protocols

References for Anti-alpha 1 Fetoprotein antibody (ab54745)

ab54745 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Leica's universal IHC blockig/diluent solution as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Sample Cat Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Formaldehyde
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Submitted Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"