Anti-alpha 1 Fetoprotein antibody (ab8202)

Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Goat polyclonal to alpha 1 Fetoprotein
  • SpecificitySpecificity of the antisera is confirmed by ELISA (OPD) on human alpha-Fetoprotein versus albumin, IgG, IgM. Tests with immunoblotting of fetal and adult sera. Ouchterlony double diffusion, immunoelectrophoresis. No crossreactivity with human plasma proteins.
  • Tested applicationsSuitable for: WB, ELISA, Agglutinationmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

Properties

  • FormLyophilised:Please reconstitute the vial with 1ml sterile distilled water
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferConstituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antiserum was subjected to absorption with immobilized human albumin fraction and globulin fractions and fibrinogen in order to remove non-specific antibodies. The affinity purified antibody is obtained by binding to immobilized human alpha-Fetoprotein (antigen used for immunization), followed by elution with acidic buffer, titration, dialysis, dispensing and lyophilization.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
AP
Agglutination
  • Application notesRecommended for immunoblotting. Sandwich-ELISA with monoclonal antibody to AFP (ab8198 and ab8199 sensitivity 0.5 ng/ml). Affinity chromatography with antibody-sorbent for isolation of 85% pure AFP. Latex coating for APF agglutination assay.
  • Target

    • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
    • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
    • Post-translational
      modifications
      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Afp antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    References for Anti-alpha 1 Fetoprotein antibody (ab8202)

    ab8202 has not yet been referenced specifically in any publications.

    Product Wall

    I am sorry for the inconvenience caused by this import restriction. While ab3980 is our only Anti-alpha 1 Fetoprotein raised in goat, we do offer a number of other Anti-alpha 1 Fetoprotein. We would recommend use of ab3980, Mouse monoclonal [AFP-01] to...

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    Thank you for your request. This product (Alpha Fetoprotein antibody Ref:ab8202) has not yet been tested for cross-reactivity with mouse AFP, therefore we cannot guarantee results.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"