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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-alpha 1 Fetoprotein antibody [AFP-11]
See all alpha 1 Fetoprotein products (31) ...
Description
Mouse monoclonal [AFP-11] to alpha 1 Fetoprotein
Specificity
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Tested applications
ICC/IF, ELISA, IHC-P, RIA, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified) (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by precipitation methods.
Clonality
Monoclonal
Clone number
AFP-11
Isotype
IgG1
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Liver development
Cancer >> Tumor biomarkers >> Tumor antigens
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200(PubMed: 19497803)
ELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein (ab3980).
IHC-P: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein (ab3980). (Can be used as Capture antibody.)
Target
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac.
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational
modifications
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFP antibody
- Alpha fetoglobulin antibody
- Alpha fetoprotein antibody
see all
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
References for Anti-alpha 1 Fetoprotein antibody [AFP-11] (ab3969)
This product has been referenced in:
- Moore RN & Moghe PV Expedited growth factor-mediated specification of human embryonic stem cells toward the hepatic lineage. Stem Cell Res : (2009). ICC/IF; Human.Read more (PubMed: 19497803) »
- Stefanova Iet al. Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods 111:67-73 (1988).Read more (PubMed: 2455753) »
See all 2 publications for this product
Publishing research using ab3969? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"