Anti-alpha 1 Fetoprotein antibody [AFP-11] (ab3969)

Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody [AFP-11]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Mouse monoclonal [AFP-11] to alpha 1 Fetoprotein
  • SpecificityThe antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, RIA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.

Properties

Applications

Our Abpromise guarantee covers the use of ab3969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. PubMed: 19497803
IHC-P Use a concentration of 10 µg/ml.
RIA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody [AFP-11] images

  • Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma (fetal liver) sections labelling alpha 1 Fetoprotein with ab3969 at 10 µg/mL. 

References for Anti-alpha 1 Fetoprotein antibody [AFP-11] (ab3969)

This product has been referenced in:
  • Moore RN & Moghe PV Expedited growth factor-mediated specification of human embryonic stem cells toward the hepatic lineage. Stem Cell Res 3:51-62 (2009). ICC/IF ; Human . Read more (PubMed: 19497803) »
  • Stefanova I  et al. Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods 111:67-73 (1988). Read more (PubMed: 2455753) »

See all 2 Publications for this product

Product Wall

Having reviewed our catalog of anti-alpha 1 Fetoprotein antibodies suitable for use in ELISA, I have found the following 2 pairs which have been verified by us. AB8276 pairs with ab37207. More information about these items may be found at: Ab8276...

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