Mouse monoclonal [C3] to alpha 1 Fetoprotein, prediluted
SpecificityAlpha-Fetoprotein (AFP) is a single chain glycoprotein of 70 kDa that is normally expressed in the fetal liver, gastrointestinal tract and yolk sac.
This antibody recognizes an epitope of alpha - Fetoprotein present in human and several other species.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
Sequence similaritiesBelongs to the ALB/AFP/VDB family. Contains 3 albumin domains.
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated.