Recombinant
RabMAb

Anti-alpha 1 Fetoprotein antibody [EP1016Y] (ab52940)

Overview

  • Product name
    Anti-alpha 1 Fetoprotein antibody [EP1016Y]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Rabbit monoclonal [EP1016Y] to alpha 1 Fetoprotein
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminal of human alpha 1 Fetoprotein.

  • Positive control
    • HepG2 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-Fr or IHC-P.
  • Target

    • Function
      Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    • Tissue specificity
      Plasma. Synthesized by the fetal liver and yolk sac.
    • Sequence similarities
      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Developmental stage
      Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
    • Post-translational
      modifications
      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Afp antibody
      • AFPD antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    Images

    • Anti-alpha 1 Fetoprotein antibody [EP1016Y] (ab52940) at 1/500000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References

    This product has been referenced in:
    • Venkataramani V  et al. Amyloid precursor protein is a biomarker for transformed human pluripotent stem cells. Am J Pathol 180:1636-52 (2012). WB ; Human . Read more (PubMed: 22305861) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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