Overview

  • Product nameAnti-alpha 1 Fetoprotein antibody [EP1017Y]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Rabbit monoclonal [EP1017Y] to alpha 1 Fetoprotein
  • Tested applicationsSuitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human alpha 1 Fetoprotein (C terminal).

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab45147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 69 kDa.
IHC-P Use at an assay dependent concentration.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
    • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
    • Post-translational
      modifications
      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Afp antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    Anti-alpha 1 Fetoprotein antibody [EP1017Y] images

    • Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147) at 1/50000 dilution + HepG2 membrane

      Predicted band size : 69 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Ab45147 staining human alpha 1 Fetoprotein in human fetal liver by immunohistochemistry using paraffin embedded tissue.

    References for Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)

    This product has been referenced in:
    • Joshi M  et al. Chemokine-mediated robust augmentation of liver engraftment: a novel approach. Stem Cells Transl Med 4:21-30 (2015). Read more (PubMed: 25473087) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"