Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)


  • Product name
    Anti-alpha 1 Fetoprotein antibody [EPR9309]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Rabbit monoclonal [EPR9309] to alpha 1 Fetoprotein
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human alpha 1 Fetoprotein.

  • Positive control
    • Human plasma, HepG2 and Human fetal liver lysates, Human moderately differentiated hepatocellular carcinoma and Human poorly differentiated hepatocellular carcinoma tissues, HepG2 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab169552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/50.


  • Function
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity
    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all


  • All lanes : Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 68 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin embedded Human moderately differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with ab169552 antibody at 1/50.
  • Immunofluorescent analysis of HepG2 cells labeling alpha 1 Fetoprotein with ab169552 at 1/100.
  • Immunoprecipitation. ab169552 at 1/1000 staining alpha 1 Fetoprotein in HepG2 cell lysate immunoprecipitated using ab169552 at 1/10. Predicted band size : 68 kDa.
  • Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling alpha 1 Fetoprotein with purified ab169552 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Immunohistochemical analysis of paraffin embedded Human poorly differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with ab169552 antibody at 1/50.


This product has been referenced in:
  • Yanagi Y  et al. In vivo and ex vivo methods of growing a liver bud through tissue connection. Sci Rep 7:14085 (2017). IHC ; Rat . Read more (PubMed: 29074999) »
  • Xin H  et al. Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts. PLoS One 9:e85308 (2014). IHC-P ; Human . Read more (PubMed: 24416385) »

See all 2 Publications for this product

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