Overview

  • Product nameAnti-alpha 1 Glycine Receptor antibody
    See all alpha 1 Glycine Receptor primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Glycine Receptor
  • SpecificityReacts with Human and Rat a1 and a2 glycine receptor subunits.
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    ARSATKPMSPSDFLDKLMGC

    conjugated to KLH, corresponding to N terminal amino acids 29-47 of Human alpha 1 Glycine Receptor.

  • Positive control
    • Spinal cord homogenate
  • General notesLyophilized from ammonium bicarbonate.

Properties

Applications

Our Abpromise guarantee covers the use of ab475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-Fr
WB
  • Application notesWB: 1/1000.
    IHC-Fr: 1/1000. We have no further details on the protocol used.
    ICC/IF: 1/10 (from pubMed:17308032). Incubate cells with primary antibody for 30m ins at 37C, then wash and fix with 4% PFA in PBS for 10 mins, then wash and incubate with secondary antibody.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
    • Involvement in diseaseDefects in GLRA1 are a cause of startle disease (STHE) [MIM:149400]; also known as hereditary hyperekplexia or congenital stiff-person syndrome. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. Inheritance can be autosomal dominant or recessive.
    • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily.
    • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GLRA1 antibody
      • GLRA1_HUMAN antibody
      • Glycine receptor 48 kDa subunit antibody
      • Glycine receptor alpha 1 antibody
      • Glycine receptor strychnine-binding subunit antibody
      • Glycine receptor subunit alpha-1 antibody
      • Glycine receptor, alpha 1 subunit antibody
      • HKPX1 antibody
      • STHE antibody
      see all

    References for Anti-alpha 1 Glycine Receptor antibody (ab475)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    I am waiting for information about the immunogen for ab23809 which should allow us to predict if the antibody will cross-react with alpha 3. You might also want to consider the antibody ab475; a BLAST search on the immunogen (there is a link on the dat...

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    I can confirm that the antibody has been tested in IHC frozen section. The IHC frozen testing was done by a customer, however, and we do not have the specific information on how it was optimized. I would suggest trying both 4% PFA fixation and aceton...

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    Thank you for your recent phone call. I enclose below the protocol used in the reference Caraiscos VB et al. Insulin increases the potency of glycine at ionotropic glycine receptors. Mol Pharmacol 71:1277-87 (2007). PubMed: 17308032 "Immunocytoche...

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    Thank you for your email. Ab475 is a rabbit polyclonal antibody and the isotype is IgG. However, we do not carry a rabbit IgG isotype control at this time. To see if another company may have what you are looking for, you can click on the link to "T...

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    Thank you for your e-mail. To our knowledge, this antibody has not been tested for immunofluorescence studies. It has only been characterized for Western blot and IP applications. If you decide to go ahead and purchase this product, please let u...

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    Thank you for your enquiry and your interest in our products. Adult glycine receptors are heterodimers composed of two subunits of 48 kDa and 58 kDa, denoted as a1 and b subunits. Foetal glycine receptors are homomers composed of a2 subunits. Thi...

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    The synthetic peptide sequence was ARSATKPMSPSDFLDKLMGC.

    Dear Sir: This antibody is specific for rat spinal cord and cell extracts that contain recombinant human glycine receptors. You may want to check the following references; Rajendra, S., et al, Pharmacol. Ther., 73:m121-146 (1997) AND Raymon...

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    Unfortunately, it is not suitable for that application.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"