Anti-Alpha 1 microglobulin antibody [BN11.10] (ab18513)


  • Product nameAnti-Alpha 1 microglobulin antibody [BN11.10]
    See all Alpha 1 microglobulin primary antibodies
  • Description
    Mouse monoclonal [BN11.10] to Alpha 1 microglobulin
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, IP, RIA, Othermore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length native protein (purified) (Human and rat).



Our Abpromise guarantee covers the use of ab18513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 39 kDa.
ELISA Use a concentration of 0.2 - 2 µg/ml.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.


  • FunctionInter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • Tissue specificityExpressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • Sequence similaritiesIn the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • Post-translational
    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1M antibody
    • Alpha 1 microglobulin/bikunin precursor antibody
    • Alpha 1 microglycoprotein antibody
    • Alpha-1 microglycoprotein antibody
    • Alpha-1-microglobulin antibody
    • AMBP antibody
    • AMBP_HUMAN antibody
    • Bikunin antibody
    • Complex-forming glycoprotein heterogeneous in charge antibody
    • EDC1 antibody
    • Growth inhibiting protein 19 antibody
    • HCP antibody
    • HI-30 antibody
    • HI30 antibody
    • IATIL antibody
    • ITI antibody
    • ITI-LC antibody
    • ITIL antibody
    • Microglobulin (alpha 1) antibody
    • Protein AMBP antibody
    • Protein HC antibody
    • Trypstatin antibody
    • Uristatin antibody
    • Uronic-acid-rich protein antibody
    • UTI antibody
    see all

Anti-Alpha 1 microglobulin antibody [BN11.10] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal liver tissues (left) and ICC tissues (right) labelling Alpha 1 microglobulin with ab18513 at 1/200. Alpha 1 microglobulin positive cells were mainly detected in the cytosol of hepatocytes in normal liver tissues and peri-tumoral tissues, but not in ICC tissues. T, ICC tissue; N, peri-tumoral tissue. Magnifications: 200X.

References for Anti-Alpha 1 microglobulin antibody [BN11.10] (ab18513)

This product has been referenced in:
  • Wang AG  et al. Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. Biochem Biophys Res Commun 345:1022-32 (2006). IHC-P ; Human . Read more (PubMed: 16712791) »

See 1 Publication for this product

Product Wall

The immunogen used to produce Anti-Alpha 1 microglobulin antibody [BN11.10] cat# ab18513 is human and rat alpha-1-microglobulin, native, full length, purified from urine.
I confirm it corresponds to the alpha-1-microglobulin fragment: UniProt...

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Thank you for contacting us. Indeed, the Alpha 1 microglobulin antibody ab18513 has been tested in an indirect ELISA protocol. Please find attached our detailed protocol. Protocols for other ELISA techniques as well as other applications can also...

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