alpha 1 Microglobulin Human ELISA Kit (ab108884)

Overview

  • Product namealpha 1 Microglobulin Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.6 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    97.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha 1 Microglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Microglobulin concentrations in Human cell culture supernatants, serum, plasma, urine, saliva, CSF and milk.

    An alpha 1 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 Microglobulin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

alpha 1 Microglobulin Human ELISA Kit images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample

Protocols

References for alpha 1 Microglobulin Human ELISA Kit (ab108884)

ab108884 has not yet been referenced specifically in any publications.

Product Wall



Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfami...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"