Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)

Overview

  • Product nameAnti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker
    See all alpha 1 Sodium Potassium ATPase primary antibodies
  • Description
    Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase - Plasma Membrane Marker
  • SpecificityThis antibody is specific for Na,K-ATPase alpha 1 subunit.
  • Tested applicationsIHC-P, IHC - Wholemount, ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Full length native protein (purified) (Rabbit renal outermedulla).

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
  • General notes

    This product is available conjugated to FITC. Please see ab150007. This antibody has been used as a plasma membrane marker.  This antibody is available as an azide-free product (see ab174786).

Properties

Applications

Our Abpromise guarantee covers the use of ab7671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
Flow Cyt Use a concentration of 10 µg/ml. See Abreview.

Target

  • FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications
    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC1798 antibody
    • MGC25056 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker images

  • Lanes 1 - 3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1 µg/ml
    Lanes 4 - 6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 2 µg/ml
    Lanes 7 - 9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 5 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 6 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 8 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 9 : Brain (Rat) Tissue Lysate (ab7942)

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa


    Exposure time : 3 minutes
  • All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
    Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
    Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate

    Predicted band size : 112 kDa
  • ab7671 staining (dog) MDCK II cells by ICC/IF.  Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C.  Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.

    See Abreview

  • ab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The  sample was incubated with the primary antibody 10 µg/ml in PBS  for 1 hour. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.

    See Abreview

  • ab7671 staining Sodium Potassium ATPase in 293 human embryonic kidneys cells. Cells were plated on an uncoated cover slip. One day after plating cells were washed twice with PBS and fixed with 4% paraformaldehyde with 4% sucrose at room temperature for 15 minutes. After washing once with PBS, 300µl wheat germ agglutinin (5µg/ml in PBS) conjugated to TexasRed were added and incubated at 4°C in darkness for 15 minutes. Cells were then washed twice with PBS and permeabilized with 0.5% saponin in PBS for 45 minutes at 4°C. Blocking was carried out with 10% fetal calf serum in PBS-saponin during first and second antibody incubation. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/500 dilution. After additional washing cover slips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI.

    See Abreview

  • IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab7671 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7671, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.
  • All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671) at 1/1000 dilution

    Lane 1 : Parotid acinar cell lysate
    Lane 2 : Parotid acinar cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-Mouse polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 112 kDa


    Exposure time : 5 minutes

    See Abreview

References for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (ab7671)

This product has been referenced in:
  • Müller J  et al. O-fucosylation of the notch ligand mDLL1 by POFUT1 is dispensable for ligand function. PLoS One 9:e88571 (2014). ICC/IF ; Chinese Hamster . Read more (PubMed: 24533113) »
  • Zhang Y  et al. A systems biology-based investigation into the therapeutic effects of Gansui Banxia Tang on reversing the imbalanced network of hepatocellular carcinoma. Sci Rep 4:4154 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24561634) »

See all 49 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Hepatocytes)
Specification Hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 30 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (kidney)
Specification kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 30 2014

Application IHC - Wholemount
Sample Human Tissue (Beas-2B)
Specification Beas-2B
Username

Mr. Brad Howard

Verified customer

Submitted Apr 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Caco^2 cell)
Specification Caco^2 cell
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain sections (cortex))
Specification mouse brain sections (cortex)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Tri-Sodiumcitrate Dihydrate, pH6.0
Permeabilization Yes - Triton during staining
Blocking step PBS, 1%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 04 2013


The lab let me know that we don't have more information about the immunogen for this antibody than what is on the datasheet. The only information we have is that the antibody reacts with alpha 1 subunit. There's no mention of it reacting with othe...

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Application Western blot
Sample Human Cell lysate - other (HeLa lysate - post nuclear supernatant)
Loading amount 20 µg
Specification HeLa lysate - post nuclear supernatant
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Mixed glial culture)
Specification Mixed glial culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 19 2012

Thank you for your reply. The protocol you are using seems fine. My only recommendation would be that it may help to use 0.1% Tween 20 or Saponin for permeabilization instead of Triton X-100. Tween and Saponin will open pores in the plasma membrane, bu...

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Thank you for your enquiry regardingab7671 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise app...

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