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Full length native protein (purified) (Rabbit renal outermedulla).
Our Abpromise guarantee covers the use of ab7671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|IHC - Wholemount||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.|
|Flow Cyt||Use a concentration of 10 µg/ml. See Abreview.|
ab7671 staining (dog) MDCK II cells by ICC/IF. Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.
ab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody 10 µg/ml in PBS for 1 hour. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.
ab7671 staining Sodium Potassium ATPase in 293 human embryonic kidneys cells. Cells were plated on an uncoated cover slip. One day after plating cells were washed twice with PBS and fixed with 4% paraformaldehyde with 4% sucrose at room temperature for 15 minutes. After washing once with PBS, 300µl wheat germ agglutinin (5µg/ml in PBS) conjugated to TexasRed were added and incubated at 4°C in darkness for 15 minutes. Cells were then washed twice with PBS and permeabilized with 0.5% saponin in PBS for 45 minutes at 4°C. Blocking was carried out with 10% fetal calf serum in PBS-saponin during first and second antibody incubation. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/500 dilution. After additional washing cover slips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI.
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